Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand |
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| Authors: | Patharapol Piamsomboon Tidaporn Chaweepack Jee Eun Han Kathy Fengjyu Tang Janenuj Wongtavatchai |
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| Affiliation: | 1. Faculty of Veterinary Science, Prince of Songkla University, Songkhla, Thailand;2. Department of Fisheries, Chanthaburi Coastal Fisheries Research and Development Center, Ministry of Agriculture and Cooperative, Chanthaburi, Thailand;3. Advanced Research Center, CJ CheilJedang Feed & Livestock Research Institute, Suwon, Korea;4. ShrimpVet Laboratory, Ho Chi Minh City, Vietnam;5. Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand |
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| Abstract: | White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the shrimp farming industry in Thailand. Genotyping analysis was done in 124 WSSV isolates from cultured Pacific white shrimp Litopenaeus vannamei. These samples were obtained during 2007–2014 from eight provinces in Thailand. We investigated five variable loci in the virus genome: deletions in two variable regions, VR14/15 and VR23/24, and three variable number tandem repeats (VNTR) located in open reading frame (ORF) 75, 125 and 94. WSSV genotype was characterized as (X14/15, X23/24) (N75‐N125‐N94) where X is the number of base pair deletion in the variable region and N is the number of repeat units (RUs) in a specific ORF. The deletion pattern in VR14/15 and VR23/24 regions characterized three WSSV genotypes. The most prevalent genotype was (595014/15, 1097123/24), and it was found in all studied areas. At least 33 genotypes of WSSV were analysed based on 3 VNTR loci, indicating that the VNTRs of WSSV genome are highly variable. From 124 WSSV samples, two samples presented the characteristic of all five variable loci similar to WSSV collected during 2010 in Saudi Arabia (595014/15, 1097123/24) (375‐6125‐794). Many different WSSV genotypes shown in this study as compared to previously reported genotypes in Thailand suggests current status of disease epidemiology, as well as probable movements of WSSV between countries. |
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| Keywords: |
Litopenaeus vannamei
variable loci virus genotyping white spot disease |
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