Pearl grafting: Tracking the biological origin of nuclei by straightforward immunological methods |
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| Authors: | Nelly Schmitt Frédéric Marin Jérôme Thomas Laurent Plasseraud Marina Demoy‐Schneider |
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| Affiliation: | 1. UMR 241 Ecosystèmes Insulaires Océaniens, Equipe Etude Intégrée des Métabolites Secondaires (EIMS), Université de la Polynésie fran?aise, Tahiti, French Polynesia;2. Laboratoire CNRS UMR 6282 ?Biogéosciences?, Université de Bourgogne ‐ Franche Comté (UB‐FC), Dijon, France;3. Institut de Chimie Moléculaire de l'Université de Bourgogne (ICMUB), UMR CNRS 6302, Université de Bourgogne ‐ Franche Comté (UB‐FC), Dijon Cedex, France |
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| Abstract: | French Polynesia is renowned for the production of Tahitian black pearl. These gems are obtained by grafting a nucleus into the gonad of a receiving oyster together with a graft, i.e. a small section of mantle tissue of a donor oyster. This procedure initiates the formation of a pearl sack around the nucleus, and subsequently, the deposition of concentric layers of nacre. The nucleus plays a key‐role in pearl formation and its characteristics influence markedly the quality of the final product. As it is manufactured from mollusc shells, it contains a small percentage of organics. In the present paper, we used a set of biochemical techniques to characterize and compare the organic matrices from two types of nuclei that are currently used in French Polynesia: that from the freshwater mussel Amblema sp., and that from the pearl oyster Pinctada sp. To this end, we extracted the matrices from nuclei and performed FT‐IR, monodimensional electrophoresis, and enzyme‐linked immuno‐sorbent assay (ELISA). Our data show that the matrix associated with Amblema nuclei has a very different biochemical signature from that of Pinctada nuclei, a fact that may explain the improved tolerance of grafted oysters to nuclei of Pinctada origin. In the absence of complex physical methods of investigation, simple immunological techniques and FT‐IR performed on the extracted organic matrix are extremely reliable and effective for discriminating nuclei from these two sources. We assert that such techniques can be used as a diagnostic test to track unambiguously the biological origin of nuclei to avoid fraud. |
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| Keywords: |
Amblema plicata
ELISA matrix proteins nucleus pearl oyster
Pinctada margaritifera
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