| 山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定 |
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| 引用本文： | 邢会琴,王春明,金社林,周天旺,郭成. 山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定[J]. 草地学报, 2021, 29(6): 1350-1356. DOI: 10.11733/j.issn.1007-0435.2021.06.027 |
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| 作者姓名： | 邢会琴 王春明 金社林 周天旺 郭成 |
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| 作者单位： | 1. 河西学院农业与生态工程学院, 甘肃 张掖 734000;2. 甘肃省农业科学院植物保护研究所, 甘肃 兰州 730070 |
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| 基金项目： | 绿肥产业技术体系旱地病虫害综合防控岗位（CARS-22-G-20）；甘肃省科技计划（20JR5RA108）；嘉峪关市科技计划（20-17）资助 |
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| 摘 要： | 为了明确山黧豆(Lathyrus sativus)根腐病病原菌的分类地位,本研究于2018年8月在四川省南充市采集山黧豆根腐病病样,经组织分离法进行分离纯化,采用形态学和翻译延伸因子(Translation elongation factor 1-agene,EF-1α)基因序列分析相结合的方法对致病菌进行鉴定.结果表...
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| 关 键 词： | 山黧豆 根腐病 亚洲镰孢菌 病原鉴定 EF-1α |
| 收稿时间： | 2020-12-12 |
Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot |
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| XING Hui-qin,WANG Chun-ming,JIN She-lin,ZHOU Tian-wang,GUO Cheng. Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot[J]. Acta Agrestia Sinica, 2021, 29(6): 1350-1356. DOI: 10.11733/j.issn.1007-0435.2021.06.027 |
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| Authors: | XING Hui-qin WANG Chun-ming JIN She-lin ZHOU Tian-wang GUO Cheng |
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| Affiliation: | 1. College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu Province 734000, China;2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China |
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| Abstract: | In order to identify the pathogen causing root rot on Lathyrus sativus,the infected plants were collected during August of 2018 in Nanchong city,Sichuan province. The pathogen was isolated from tissue， single spore was purified,and pathogenicity was tested in the laboratory. Pathogenic isolates were identified by morphological characteristics and EF-1α gene sequence analysis. The results showed that 39 isolates were obtained from infected plant,of which three isolates including Shan-27,Shan-28 and Shan-29 had strong pathogenicity to L.sativus,accounting for 7.69%. The above three strains were all close to Fusarium graminearum species complex in morphology. Their translation elongation factor (EF-1α) were amplified by PCR,sequenced,and compared with Blast in GenBank. The results showed that their EF-1α were clustered in the same group with F. asiaticum, and their homology were more than 99%. Therefore,according to the morphological characteristics of the isolates and the sequence analysis of EF-1α gene,the three pathogenic isolates causing L.sativus root rot were identified as F. asiaticum. |
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| Keywords: | Lathyrus sativus Root rot Fusarium asiaticum Identification of pathogen Translation elongation factor |
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