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| 盐胁迫下转Bt基因棉的K+、Na+转运及SOD活性的变化 | |
| 引用本文： | 张敏,王校常,严蔚东,梁永超,施卫明. 盐胁迫下转Bt基因棉的K+、Na+转运及SOD活性的变化[J]. 土壤学报, 2005, 42(3): 460-467 |
| 作者姓名： | 张敏 王校常 严蔚东 梁永超 施卫明 |
| 作者单位： | 1. 南京农业大学资源与环境科学学院,南京,210095;土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008 2. 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008 3. 南京农业大学资源与环境科学学院,南京,210095 |
| 基金项目： | 国家自然科学基金项目(30270789)、中国科学院南京土壤研究所知识创新工程资助 |
| 摘 要： | 通过对转Bt基因棉(苏抗103)和受体棉(苏棉12)在不同浓度盐胁迫条件下的钾钠离子的转运、累积分布以及它们SOD活性和H2O2含量变化的比较,证明了Bt抗虫基因的导入一方面导致棉花植株地上部钾累积增多,根部钾含量降低,从根往地上部的钾转运能力增强,而且根吸收能力也有增强趋势;然而另一方面,受盐胁迫时转Bt基因棉对钾的向上运输选择性减弱,地上部钠积累偏多,盐耐性减弱;SOD活性受盐胁迫影响,下降显著,超氧自由基的清除能力可能受到影响。结果表明,与常规棉有所不同,转Bt基因棉可能不太适宜在盐碱土壤上种植。 |
| 关 键 词： | 转Bt基因棉 盐胁迫 超氧自由基 钾吸收转运 |
| 收稿时间： | 2004-05-05 |
| 修稿时间： | 2004-09-06 |
K+ AND Na+ UPTAKE AND TRANSPORT AND SOD ACTIVITY IN Bt TRANSGENIC COTTON SEEDLINGS UNDER SALT STRESS |
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| Zhang Min,Wang Xiaochang,Yan Weidong,Liang Yongchao and Shi Weiming. K+ AND Na+ UPTAKE AND TRANSPORT AND SOD ACTIVITY IN Bt TRANSGENIC COTTON SEEDLINGS UNDER SALT STRESS[J]. Acta Pedologica Sinica, 2005, 42(3): 460-467 | |
| Authors: | Zhang Min Wang Xiaochang Yan Weidong Liang Yongchao Shi Weiming |
| Affiliation: | College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China |
| Abstract: | The present paper addresses changes in uptake, transport and allocation of potassium and sodium, SOD (superoxide dismutases) activity and H_2O_2 content in leaves of Bt (Bacillus thuringiensis) transgenic cotton (Sukang 103) induced by different levels of salt stress as compared with those in its conventional parent (Sumian 12) at the early growth stage. The results showed that after receiving Bt gene the cotton increased its demand for potassium; more potassium was accumulated in shoot while the potassium content in roots was lowered as compared with the regular cotton. It seems to indicate that potassium transport ability is enhanced. On the other hands, when both regular and Bt cotton seedlings were exposed to salt stress, the ability of selecting potassium for transport from root to shoot was weakened in the Bt transgenic cotton, consequently more sodium was accumulated in the shoot and salt tolerance was lower than that in the control. Furthermore, SOD activity in leaves of the Bt cotton decreased significantly under salt stress, which might lead to reduced ability of eliminating superoxide radicals (O-_2). Results of the research suggest that Bt cotton is more sensitive to salt stress than regular cotton and may not be suitable for cultivation in saline soils. |
| Keywords: | Bt transgenic cotton Salt stress Superoxide radicals Absorption and transport of potassium |
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