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| 乏情和发情初产母猪卵巢microRNA差异表达分析 | |
| 引用本文： | 任巧玲,白献晓,王璟,郭红霞,陈俊峰,马文涛,和小娥,张家庆,邢宝松. 乏情和发情初产母猪卵巢microRNA差异表达分析[J]. 中国畜牧兽医, 2018, 45(5): 1153-1162. DOI: 10.16431/j.cnki.1671-7236.2018.05.004 |
| 作者姓名： | 任巧玲 白献晓 王璟 郭红霞 陈俊峰 马文涛 和小娥 张家庆 邢宝松 |
| 作者单位： | 1. 河南省农业科学院畜牧兽医研究所, 郑州 450002; 2. 河南省动物卫生监督所, 郑州 450008; 3. 郑州市动物卫生监督所, 郑州 450000 |
| 基金项目： | 河南省科技攻关计划项目（162102110036）；河南省农业科学院优秀青年科技基金（2016YQ19） |
| 摘 要： | 为了探索卵巢microRNA （miRNA）在初产母猪生殖调控中的作用，本研究利用Illumina高通量测序技术检测了乏情和发情初产母猪卵巢miRNA的表达谱，并对表达量较高且差异显著的miRNA进行生物信息学分析。结果显示，本研究构建的两个small RNA文库共鉴定出503个miRNAs，其中已知的303个，新预测的200个。在已知的miRNA中，ssc-miR-10b在两个文库中的表达量最高，其次为ssc-miR-143-3p和ssc-miR-26a；在新预测的miRNA中，chr13_2637_mature在两个文库中的表达量最高，其次为chr8_9994_mature。与发情母猪相比，共有145个miRNAs发生显著变化（read counts>10，∣log2（fold-change）∣>1），上调114个，下调31个。在进一步筛选的31个表达量较高且差异显著的miRNAs（read counts>1 000，∣log2（fold-change）∣>1）中，新预测的chr13_2585_mature上调倍数最高，且只在乏情母猪卵巢中表达。31个miRNAs共预测到7 388个靶基因，KEGG信号通路分析显示，有2 788个靶基因注释到了297个KEGG通路，前20个最富集的通路部分与生殖过程或生殖活动调控相关，表明这31个miRNAs参与了初产母猪的生殖调控。本研究结果丰富了猪miRNA数据资源，为进一步深入研究初产母猪的繁殖性能提供了理论依据。 |
| 关 键 词： | miRNAs 乏情 发情 初产母猪 卵巢 |
| 收稿时间： | 2017-11-02 |
Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows |
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| REN Qiaoling,BAI Xianxiao,WANG Jing,GUO Hongxia,CHEN Junfeng,MA Wentao,HE Xiaoe,ZHANG Jiaqing,XING Baosong. Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(5): 1153-1162. DOI: 10.16431/j.cnki.1671-7236.2018.05.004 | |
| Authors: | REN Qiaoling BAI Xianxiao WANG Jing GUO Hongxia CHEN Junfeng MA Wentao HE Xiaoe ZHANG Jiaqing XING Baosong |
| Affiliation: | 1. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2. Henan Animal Health Inspection Institute, Zhengzhou 450008, China; 3. Zhengzhou Animal Health Inspection Institute, Zhengzhou 450000, China |
| Abstract: | To explore the role of ovarian microRNA (miRNA) in regulating the reproductive process in primiparous sows,the miRNA expression profiles were examined using Illumina high-throughout sequencing.The miRNA with high expression and significant difference were analyzed by bioinformatics methods.The results showed that a total of 503 miRNAs were detected in two small RNA libraries,which included 303 known and 200 novel miRNAs.The 3 most highly expressed miRNAs in known miRNAs were ssc-miR-10b,ssc-miR-143-3p and ssc-miR-26a in two small RNA libraries.The highest specific expressed miRNAs in novel miRNAs was chr13_2637_mature,followed by chr8_9994_mature.Compared with estrous primiparous sows,a total of 145 differentially expressed miRNAs (read counts>10,|log2(fold-change)|>1),including 114 upregulated miRNAs and 31 downregulated miRNAs were identified.31 differentially expressed miRNAs (read counts>1 000,∣log2(fold-change)∣>1) were further screened.In these miRNAs,chr13_2585_mature was the highest-upregulated,and was discovered only in the ovary of anestrous primiparous sows.Furthermore,7 388 target genes were predicted from 31 miRNAs.KEGG pathway analyses showed that a total of 2 788 target genes were annotated to 297 pathways,and the top 20 most significantly enriched pathways were involved in reproductive processes,suggesting the 31 miRNAs played a crucial role in the reproductive process of primiparous sows.These results enriched the porcine miRNA database and provided useful information for further research about the reproductive performance of primiparous sows. |
| Keywords: | miRNAs anestrus estrus primiparous sow ovary |
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