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| 静原鸡ELOVL2基因遗传多态性研究 | |
| 引用本文： | 母童,顾亚玲,赵平,冯小芳,陈佳萍,陈亚飞,刘丽元,蒋秋斐,张娟. 静原鸡ELOVL2基因遗传多态性研究[J]. 中国畜牧兽医, 2018, 45(3): 730-737. DOI: 10.16431/j.cnki.1671-7236.2018.03.022 |
| 作者姓名： | 母童 顾亚玲 赵平 冯小芳 陈佳萍 陈亚飞 刘丽元 蒋秋斐 张娟 |
| 作者单位： | 1. 宁夏大学农学院, 银川 750021; 2. 彭阳县畜牧技术推广服务中心, 固原 756500 |
| 基金项目： | 2017年自治区级大学生创新创业训练计划项目--静原鸡ELOVL5基因的多态性研究（Q201710749050） |
| 摘 要： | 为了探讨静原鸡极长链脂肪酸延伸酶蛋白2（elongation of very-long-chain fatty acids 2,ELOVL2）基因的遗传特性,确定核苷酸的变异位点,本研究采用PCR-SSCP及DNA测序技术对静原鸡ELOVL2基因进行多态性检测。结果显示,静原鸡ELOVL2基因外显子6无多态性,内含子2和6呈中度多态。在ELOVL2基因内含子2扩增片段上共检测到5种基因型：A2A2、B2B2、C2C2、D2D2和A2C2,4种等位基因：A2、B2、C2和D2,其中A2为优势等位基因（0.62）,A2A2基因型为优势基因型（0.54）;3个SNPs位点：g.63372228+4918G > A的转换、g.63372228+5024T > C的转换和g.63372228+4928C > A的颠换。在E2e6I6扩增片段上共检测到3种基因型：A1A1、B1B1和A1B1,2种等位基因：A1和B1,其中A1为优势等位基因（0.67）,A1A1基因型为优势基因型（0.54）,等位基因B1存在g.63388000+748G > C的颠换。群体遗传学分析发现,静原鸡ELOVL2基因内含子2、6遗传纯合度均较高,且偏离了Hardy-Weinberg平衡（P < 0.05）。本研究结果表明,静原鸡ELOVL2基因内含子2和6序列突变较高,且呈中度多态,表现出纯合子优势,外显子6上无突变。 |
| 关 键 词： | 静原鸡 ELOVL2基因 多态性 |
| 收稿时间： | 2017-09-15 |
Study on Polymorphism of ELOVL2 Gene in Jingyuan Chicken |
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| MU Tong,GU Yaling,ZHAO Ping,FENG Xiaofang,CHEN Jiaping,CHEN Yafei,LIU Liyuan,JIANG Qiufei,ZHANG Juan. Study on Polymorphism of ELOVL2 Gene in Jingyuan Chicken[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(3): 730-737. DOI: 10.16431/j.cnki.1671-7236.2018.03.022 | |
| Authors: | MU Tong GU Yaling ZHAO Ping FENG Xiaofang CHEN Jiaping CHEN Yafei LIU Liyuan JIANG Qiufei ZHANG Juan |
| Affiliation: | 1. College of Agriculture, Ningxia University, Yinchuan 750021, China; 2. Animal Husbandry and Technology Promotion Service Center of Pengyang County, Guyuan 756500, China |
| Abstract: | In order to study the genetic characteristics and nucleotide polymorphism sites of elongation of very-long-chain fatty acids 2 (ELOVL2) gene in Jingyuan chicken,the polymorphisms of ELOVL2 gene were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence.The results showed that there was no polymorphism of ELOVL2 gene exon 6,there were moderate polymorphism of introns 2 and 6 in Jingyuan chicken.The total of 5 genotypes(A2A2,B2B2,C2C2,D2D2 and A2C2),4 alleles(A2,B2,C2 and D2) and 3 SNPs loci were detected on the intron 2 amplified fragment of ELOVL2 gene.The 3 SNPs were g.63372228+4918G > A transformation,g.63372228+5024T > C transformation and g.63372228+4928C > A transversion.A2 was the dominant allele (0.62),and A2A2 was the dominant genotype (0.54).The total of 3 genotypes (A1A1,B1B1 and A1B1) and 2 alleles (A1 and B1) were detected on E2e6I6 amplified fragments.A1 was the dominant allele (0.67),A1A1 was the dominant genotype (0.54),and only allele B1 had a g.63388000+748G > C transversion.Population genetics analysis showed that genetic homozygosity of ELOVL2 gene introns 2 and 6 in Jingyuan chicken were higher and deviation from Hardy-Weinberg equilibrium (P < 0.05).The results showed that the mutations of the ELOVL2 gene introns 2 and 6 in the Jingyuan chicken were high,which were moderate polymorphism,showing the homozygous advantage and no mutation on the exon 6. |
| Keywords: | Jingyuan chicken ELOVL2 gene polymorphism |
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