| 不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征 |
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| 引用本文： | 刘海洋,王伟,张仁福,温切木·阿布列孜,姚举. 不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征[J]. 新疆农业科学, 2021, 58(3): 522-531. DOI: 10.6048/j.issn.1001-4330.2021.03.015 |
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| 作者姓名： | 刘海洋 王伟 张仁福 温切木·阿布列孜 姚举 |
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| 作者单位： | 1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091; 2.阿勒泰市菜篮子工程办公室, 新疆阿勒泰836500 |
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| 基金项目： | 新疆维吾尔自治区科技支疆项目计划(2019E0244);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2020015);自治区公益性科研院所基本科研业务费(KY201807);国家重点研发计划(2017YFD0201106,2017YFD0201903) |
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| 摘 要： | [目的]土壤中大丽轮枝菌的微菌核是棉花黄萎病发生的关键因子,研究外界因素对土壤中微菌核数量的影响特征,为棉花黄萎病的防治提供参考意义.[方法]利用选择性分离培养与荧光定量PCR技术,检测采自新疆棉花黄萎病田内不同处理组土壤中的微菌核,并结合室内盆栽试验进行验证.[结果]大丽轮枝菌微菌核在棉田土壤中呈聚集分布,不同抗病性...
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| 关 键 词： | 大丽轮枝菌 微菌核 棉花黄萎病 选择性分离 根围土壤 |
| 收稿时间： | 2020-09-05 |
Quantitative Characteristics of Microsclerotia of Verticillium dahliae in Cotton Field Soil under Different Factors |
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| LIU Haiyang,WANG Wei,ZHANG Renfu,Wenqiemu Abulizi,YAO Ju. Quantitative Characteristics of Microsclerotia of Verticillium dahliae in Cotton Field Soil under Different Factors[J]. Xinjiang Agricultural Sciences, 2021, 58(3): 522-531. DOI: 10.6048/j.issn.1001-4330.2021.03.015 |
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| Authors: | LIU Haiyang WANG Wei ZHANG Renfu Wenqiemu Abulizi YAO Ju |
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| Affiliation: | 1. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China; 2. The Shopping Basket Program Office of Altay, Altay Xinjiang 836500, China |
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| Abstract: | 【Objective】 Microsclerotia of Verticillium dahliae in soil is the basis of cotton Verticillium wilt. This project aims to clarify the influence of external factors on microsclerotia in soil and provide a solution for the prevention and control of the disease.【Method】 The microsclerotia in soils collected from cotton field with Verticillium wilt under different treatments were detected using selective isolation and culture technology, and verified by indoor pot experiment.【Result】 Microsclerotia were aggregated in the soil of cotton field. Its number in the cotton rhizosphere was not significantly correlated with its disease resistance. Comparing cotton with upland rice, at the flowering and boll-forming stages, the number of microsclerotia in the cotton rhizosphere was significantly higher than that in the rice rhizosphere. At the harvest stage, the number of microsclerotia in the rice rhizosphere increased significantly, but showed no significant difference with the number of microsclerotia in the cotton rhizosphere. Indoor pot experiment revealed no significant differences in the number of microsclerotia in the rhizosphere soil between disease-resistant varieties Zhongzhimian 2, Zhongzhimian 2 + rice treatment and susceptible variety Junmian 1, Junmian 1 + rice mixed treatment. Water flooding was performed for the soil. After 20-30 days of waterflooding, the number of microsclerotia in soil increased significantly, but decreased slightly at the 60th day. After 150 days, the number of microsclerotia increased to the highest level, which was five times as much as that in the initial stage. The dynamics of growth and decline of microsclerotia in soil under rice + waterflooding treatment were consistent with those in blank control, presenting a trend of rapid increase, then decrease and then increase. The number of microsclerotia at the 150th day was 8 times as much as that in the initial stage.【Conclusion】 Microsclerotia are aggregated in the soil of cotton field. The number of microsclerotia in the cotton rhizosphere is not significantly correlated with its disease resistance. Planting upland rice in field can delay the growth of microsclerotia in early stage. Water flooding significantly promotes the growth of microsclerotia in potted planting soil. Rice planting fails to inhibit or even fail to promote the growth of microsclerotia in soil. |
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| Keywords: | Verticillium dahliae microsclerotia Verticillium wilt selective isolation rhizosphere soil |
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