| 利用SSR标记分析栽培种花生多态性及亲缘关系 |
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| 引用本文： | 韩柱强,高国庆,韦鹏霄,唐荣华,钟瑞春. 利用SSR标记分析栽培种花生多态性及亲缘关系[J]. 作物学报, 2004, 30(11): 1097-1101 |
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| 作者姓名： | 韩柱强 高国庆 韦鹏霄 唐荣华 钟瑞春 |
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| 作者单位： | 广西农业科学院经济作物研究所，广西南宁530007 |
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| 基金项目： | SupportedbyGuangxiScienceFoundation (No .Guikejj 0 2 36 0 38andNo .Guikehui 0 34 2 0 0 3) . |
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| 摘 要： | 利用11对SSR引物对24个花生栽培品种（包括四大类型）进行PCR扩增分析，其中4对检测到明显的多态性，共检测到33个等位基因变异，每一个位点上检测到的等位变异数为5～13个，平均为8.25个。根据扩增结果可以将24个品种中的21个相互区分。供试品种间的遗传相似系数值在0.2～1.0之间，平均为0.4788。根据UPGMA聚类分析结果,供试
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| 关 键 词： | SSR 花生 多态性 遗传关系 |
| 收稿时间： | 2003-07-30 |
| 修稿时间： | 2003-12-01 |
Analysis of DNA Polymorphism and Genetic Relationships in Cultivated Peanut(Arachis hypogaea L.)Using Microsatellite Markers |
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| Abstract. Analysis of DNA Polymorphism and Genetic Relationships in Cultivated Peanut(Arachis hypogaea L.)Using Microsatellite Markers[J]. Acta Agronomica Sinica, 2004, 30(11): 1097-1101 |
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| Authors: | Abstract |
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| Affiliation: | Cash Crop Institute of Guangxi Academy of Agricultural Science, Nanning 530007, Guangxi |
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| Abstract: | Eleven SSR primer pairs were used to amplify the genomic DNA isolated from 24 peanut genotypes including all 4 market types. Four primers could detected polymorphism and produced a total of 33 alleles. The average number of alleles per locus was 8.25, and up to 13 alleles were found at one locus. The genetic similarity ( GS ) indexes among 24 cultivated peanut accessions were calculated based on the data from these SSR markers. The value of GS was varied from 0.2 to 1.0, with an average of 0.4788. The results of UPGMA indicated that the majority of 24 accessions could be divided into two groups by subspecies, and most genotypes were clustered by market types. It is no doubt that microsatellites are very useful DNA markers to analyze DNA polymorphism and genetic relationship in cultivated peanut. |
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| Keywords: | SSR Microsatellites Peanut Polymorphism Genetic relationship |
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