| 植物乳杆菌XN1904E产胞外多糖发酵条件优化 |
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| 引用本文： | 陈绮,雷文平,肖茜,姚慧,罗洁,刘成国,周辉. 植物乳杆菌XN1904E产胞外多糖发酵条件优化[J]. 乳业科学与技术, 2020, 43(3): 1-5. DOI: 10.15922/j.cnki.jdst.2020.03.001 |
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| 作者姓名： | 陈绮 雷文平 肖茜 姚慧 罗洁 刘成国 周辉 |
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| 作者单位： | 湖南农业大学食品科学技术学院,湖南 长沙 410128;湖南省食品科学与生物技术重点实验室,湖南 长沙410128;湖南南山牧业有限公司,湖南 邵阳 422512 |
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| 基金项目： | 湖南省科技计划项目（2017GK4032）；国家自然科学基金面上项目（31571811） |
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| 摘 要： | 为提高植物乳杆菌XN1904E胞外多糖产量，考察培养温度、培养基初始pH值、碳源和氮源对植物乳杆菌XN1904E胞外多糖产量的影响。通过单因素试验和正交试验确定植物乳杆菌XN1904E产胞外多糖的最佳培养条件。结果表明：最佳培养条件为培养基初始pH 6.5、培养温度37 ℃、半乳糖为最适碳源、鱼蛋白胨为最适氮源，此条件下验证实验结果表明，植物乳杆菌XN1904E胞外多糖产量为235.41 mg/L；植物乳杆菌XN1904E胞外多糖水溶液（0.2 mg/mL）对1,1-二苯基-2-三硝基苯肼自由基的清除率为27.54%，表明其具有一定的抗氧化能力。
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| 关 键 词： | 植物乳杆菌XN1904E 胞外多糖 培养条件 优化 抗氧化能力 |
Optimization of Fermentation Conditions for Exopolysaccharide Production by Lactobacillus plantarum XN1904E |
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| CHEN Qi,LEI Wenping,XIAO Qian,YAO Hui,LUO Jie,LIU Chengguo,ZHOU Hui. Optimization of Fermentation Conditions for Exopolysaccharide Production by Lactobacillus plantarum XN1904E[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2020, 43(3): 1-5. DOI: 10.15922/j.cnki.jdst.2020.03.001 |
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| Authors: | CHEN Qi LEI Wenping XIAO Qian YAO Hui LUO Jie LIU Chengguo ZHOU Hui |
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| Affiliation: | (1.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Key Laboratory of FoodScience and Biotechnology, Changsha 410128, China; 3.Hunan Nanshan Animal Husbandry Co. Ltd., Shaoyang 422512, China) |
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| Abstract: | The optimization of culture temperature, initial medium pH, and carbon and nitrogen sources for improvedproduction of extracellular polysaccharides by Lactobacillus plantarum XN1904E was carried out using one-factor-at-a-time(OFAT) and orthogonal array design methods. The results showed that the optimal culture conditions were determined asfollows: initial pH 6.5, temperature 37 ℃, galactose as the best carbon source, and fish peptone as the best nitrogen source.Under these conditions, the production of extracellular polysaccharide was 235.41 mg/L. The polysaccharide at 0.2 mg/mLhad antioxidant activity in terms of scavenging rate (27.54%) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. |
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| Keywords: | Lactobacillus plantarum XN1904E exopolysaccharide culture conditions optimization antioxidant capacity |
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