| 国内新发危险性橡胶树拟盘多毛孢叶斑病鉴定及其病原学研究 |
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| 引用本文： | 李博勋,刘先宝,时涛,蔡吉苗,郑肖兰,冯艳丽,徐春华,黄贵修. 国内新发危险性橡胶树拟盘多毛孢叶斑病鉴定及其病原学研究[J]. 热带作物学报, 2020, 41(8): 1616-1624. DOI: 10.3969/j.issn.1000-2561.2020.08.016 |
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| 作者姓名： | 李博勋 刘先宝 时涛 蔡吉苗 郑肖兰 冯艳丽 徐春华 黄贵修 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海南海口 571101 |
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| 基金项目： | 国家重点研发计划项目(2017YFC1200600) |
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| 摘 要： | 基于目前橡胶树拟盘多毛孢叶斑病在境外主要植胶国爆发流行的态势,以及对天然橡胶产业经济带来的巨大影响。笔者前期对我国海南、云南、广东等省橡胶主产区的13个农场和胶园进行了病害踏查,在我国海南省乐东县志仲镇乐东保显农场发现了疑似橡胶树拟盘多毛孢叶斑病病样。本研究将该疑似病样病原菌进行分离、纯化和致病力测定,并通过形态学和分子系统学分析,确定该病原菌为新拟盘多毛孢属（Neopestalotiopsis）的Neopestalotiopsis aotearoa Maharachch., K.D. Hyde & Crous;基础生物学特性分析表明,该病原菌菌丝生长和孢子萌发的适宜温度为28℃,孢子致死温度为50℃;适宜pH为6.5,最适碳源为蔗糖和D-葡萄糖,最适氮源为硝酸钾和大豆蛋白胨;最适培养基为PDA,培养8 d后产孢。初步明确了该病的病原及其基础生物学特性,为提前储备防治该病的相关技术措施提供理论依据。
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| 关 键 词： | 橡胶树 拟盘多毛孢叶斑病 病原鉴定 生物学特性 |
| 收稿时间： | 2020-07-23 |
Identification and Etiology of a New Dangerous Neopestalotiopsis Leaf Fall Disease of Rubber Tree (Hevea brasiliensis) in China |
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| LI Boxun,LIU Xianbao,SHI Tao,CAI Jimiao,ZHENG Xiaolan,FENG Yanli,XU Chunhua,HUANG Guixiu. Identification and Etiology of a New Dangerous Neopestalotiopsis Leaf Fall Disease of Rubber Tree (Hevea brasiliensis) in China[J]. Chinese Journal of Tropical Crops, 2020, 41(8): 1616-1624. DOI: 10.3969/j.issn.1000-2561.2020.08.016 |
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| Authors: | LI Boxun LIU Xianbao SHI Tao CAI Jimiao ZHENG Xiaolan FENG Yanli XU Chunhua HUANG Guixiu |
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| Affiliation: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China |
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| Abstract: | Based on the current outbreak and epidemic situation of a novel Neopestalotiopsis leaf fall disease of rubber trees (Hevea brasiliensis) in major rubber planting countries abroad and its great impact on natural rubber industry economy, the authors conducted a preliminary inspection on 13 farms and rubber plantations in main rubber producing areas in Hainan, Yunnan and Guangdong provinces, and found suspected disease samples in Baoxian Farm, Zhizhong Town, Ledong County, Hainan. In this study, the pathogens of the suspected disease samples were isolated, purified and the pathogenicity was determined. Through morphological and molecular phylogenetic analysis, the pathogen was identified as Neopestalotiopsis aotearoa Maharachch., K.D. Hyde & Crous. The basic biological characteristics showed that the suitable temperature for the mycelial growth and spore germination was 28℃, the lethal temperature of spores was 50℃. The optimum pH value was 6.5, the optimum carbon sources were sucrose and D-glucose, the optimum nitrogen sources were potassium nitrate and soybean peptone, and the optimum medium was PDA, which could produce spores after eight days. The pathogen and its basic biological characteristics were preliminarily identified, which would provide theoretical basis for the reserve of related technical measures for the prevention and control of the disease in advance. |
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| Keywords: | Hevea brasiliensis Neopestalotiopsis leaf fall disease pathogen identification biological characteristics |
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