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Fine mapping the QTL qFS‐chr.23, using a CSIL
Authors:Youwu Wang  Jingchuan Zhang  Guangchao Kong  Hai Lin
Affiliation:1. College of Plant Science, Tarim University, Alar, Xinjiang, China;2. College of Agronomy, Shihezi University, Shihezi, Xinjiang, China;3. Northwest Inland Region Key Laboratory of Cotton Biology and Genetics Breeding, Ministry of Agriculture, Institute of Cotton, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi, Xinjiang, China
Abstract:In an earlier advanced‐backcross quantitative trait locus (QTL) analysis of an interspecific cross of Gossypium hirsutum cv. ‘Xinluzhong 36’(‘XLZH36’) and G. barbadense cv. ‘Xinhai 21’(‘XH21’), a QTL for fibre strength in the chromosome segment introgression line IL23‐09 was analysed. Single marker analysis revealed that the markers on chro.23 were associated with fibre strength. Using composite interval mapping with the F2 population (1296 plants), a QTL for fibre strength was detected on chro. 23. The QTL explained 8.9% and 15.9% of phenotypic variances in the F2 and F2 : 3 generations, respectively. Substitution mapping suggested that the QTL was located at a physical distance of 23.4 kb between the markers BNL1414 and the single nucleotide polymorphism (SNP) locus D09_43776813 C‐G. We designated this QTL as qFS‐chr.23 (quantitative trait locus for fibre strength on chro.23). This work provides a valuable genetic resource for the breeding of high fibre quality in cotton and will facilitate future efforts for map‐based cloning.
Keywords:cotton  chromosome segment introgression lines  quantitative trait locus  fibre quality  fine mapping
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