| 外源性金属硫蛋白对中国荷斯坦奶牛血淋巴细胞凋亡/坏死及线粒体膜电位的影响 |
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| 引用本文： | 吴力专,罗佳捷,张彬,李丽立,罗锐,占今舜. 外源性金属硫蛋白对中国荷斯坦奶牛血淋巴细胞凋亡/坏死及线粒体膜电位的影响[J]. 草业学报, 2013, 22(4): 205-211. DOI: 10.11686/cyxb20130425 |
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| 作者姓名： | 吴力专 罗佳捷 张彬 李丽立 罗锐 占今舜 |
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| 作者单位： | 1.湖南农业大学动物科学技术学院, 湖南 长沙410128;2.长江大学动物科学学院, 湖北 荆州434025;3.中国科学院亚热带农业生态研究所动物生态营养与健康养殖联合实验室 农业生态工程重点实验室,湖南 长沙 410125 |
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| 基金项目： | 国家自然科学基金项目，湖南省自然科学基金重点项目，教育部高等学校博士学科点专项科研基金(博导类)项目，湖南省博士生科技创新基金项目 |
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| 摘 要： | 选取20头处于热应激环境的泌乳奶牛,随机分成A、B、C和D组4组,分别按剂量0.0(对照),4.0,8.0和16.0 mg/头静脉注射金属硫蛋白(MT),以探讨外源性MT对奶牛淋巴细胞凋亡、坏死和线粒体膜电位(ΔΨm)的影响。结果表明,注射MT后,在51~60 d时,校正标准产奶量B、C和D组均显著性高于A组(P<0.05); 35 d时C组(8 mg/头)的血淋巴细胞凋亡率显著性低于B、D组(P<0.05),但与A组无显著性差异(P>0.05),D组(16 mg/头)35 d时凋亡率达到最大,随后(50~60 d)显著性下降(P<0.05);细胞的坏死率A组50 d时较1 d时显著下降(P<0.05),C组(8 mg/头)35,50 d时显著下降(P<0.05),但60 d时开始呈上升趋势(P>0.05),而D组(16 mg/头)坏死率呈现下降趋势,且在50,60 d时均显著低于注射MT之前的血淋巴细胞坏死率;35 d时,A组的线粒体膜电位值(ΔΨm)最高,但上升速度最快的是B组(4 mg/头),在35 d时显著高于20 d时的ΔΨm (P<0.05);50 d时A组和B组的ΔΨm不但不上升,反而下降,其中A组下降的速度更快,35~50 d C组(8 mg/头)保持基本恒定,60 d略有上升,D组(16 mg/头)1~35 d时逐步上升,50~60 d时保持恒定。在60 d时,ΔΨm与注射MT浓度呈正相关。说明外源性MT不仅能提高热应激状态动物的体质,还可让泌乳母牛从热应激状态平稳的过渡到热应激损伤修复状态,若注射8~16 mg/头,能抑制南方泌乳母牛夏季热应激引起的滞后效应。
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| 关 键 词： | 金属硫蛋白 中国荷斯坦奶牛 细胞凋亡 细胞坏死 线粒体膜电位 |
Effects of exogenous metallothionein on blood lymphocytes apoptosis and mitochondrial transmembrane potential of China Holstein cows |
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| WU Li-zhuan , LUO Jia-jie , ZHANG Bin , LI Li-li , LUO Rui , ZHAN Jin-shun. Effects of exogenous metallothionein on blood lymphocytes apoptosis and mitochondrial transmembrane potential of China Holstein cows[J]. Acta Prataculturae Sinica, 2013, 22(4): 205-211. DOI: 10.11686/cyxb20130425 |
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| Authors: | WU Li-zhuan LUO Jia-jie ZHANG Bin LI Li-li LUO Rui ZHAN Jin-shun |
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| Affiliation: | 1.College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2.College of Animal Science, Yangtze University, Jingzhou 434025, China; 3.Laboratory of Animal Nutrition and Human Health and Key Laboratory of Agro-ecology, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China |
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| Abstract: | Effects of exogenous metallothionein (MT) on lymphocytes apoptosis, necrosis and mitochondrial membrane voltage (ΔΨm) of China Holstein cows were studied using 20 lactating cows in heat stress. They were randomly allocated to groups A, B, C and D. MT was supplemented at 0.0, 4.0, 8.0 and 16.0 mg/capita, respectively, by intravenous injection. Corrected milk yields in groups B, C, and D were significant higher than those in group A after being injected MT during days 51-60 (P<0.05); at 35 d, the lymphocytes apoptosis rate of group C (8 mg/capita) was significantly lower than those of groups B and D (P<0.05), but was not significantly different from group A (P>0.05). The lymphocytes apoptosis rate of group D (16 mg/capita) was the highest at 35 d (P<0.05) but significantly (P<0.05) decreased during days 50-60; Compared to 1 d, the lymphocytes necrosis rate of group A significantly (P<0.05) decreased at 50 d, group C (8 mg/capita) significantly (P<0.05) decreased at 35 d and 50 d, and began to show a rising trend at 60 d (P>0.05), while group D (16 mg/capita) significantly decreased at 50 d and 60 d. The mitochondrial membrane voltage (ΔΨm) of group A was the highest at 35 d, but the Δ Ψm of group B rose fastest at 35 d (P<0.05), and was significantly higher than at 20 d (P<0.05). The ΔΨm of groups A and B decreased at 50 d with that of group A falling fastest. The ΔΨm of group C was constant during days 35-50 but increased slightly at 60 d. The ΔΨm of group D (16 mg/capita) increased gradually during days 1-35 and remained constant during days 51-60. The lymphocytes ΔΨm and injected MT amount were positively correlated at 60 d. In conclusion, the heath of cows under heat stress can be improved, by exogenous MT injection. The injection of 8-16 mg/capita, can inhibit the lagged effects of southern summer heat stress in lactating cow. |
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| Keywords: | metallothionein China Holstein cows apoptosis necrosis mitochondrial membrane voltage |
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