| 不同发育时期大豆籽粒干物质积累的QTL动态分析 |
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| 引用本文： | 韩英鹏,滕卫丽,杜玉萍,孙德生,张忠臣,徐香玲. 不同发育时期大豆籽粒干物质积累的QTL动态分析[J]. 中国农业科学, 2010, 43(7): 1328-1338. DOI: 10.3864/j.issn.0578-1752.2010.07.002 |
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| 作者姓名： | 韩英鹏 滕卫丽 杜玉萍 孙德生 张忠臣 徐香玲 |
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| 作者单位： | （哈尔滨师范大学生命科学与技术学院） |
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| 基金项目： | 国家高技术研究发展计划(863计划)，黑龙江省教育厅面上项目，哈尔滨市科技创新人才研究专项 |
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| 摘 要： | 【目的】在不同发育时期,探索影响大豆籽粒干物质积累QTL的加性效应、上位性效应和环境互作效应及其对大豆籽粒干物质积累的影响,可加深大豆育种工作者对产量形成的理解和加速育种进程。【方法】以美国大豆品种Charleston为母本,东农594为父本及二者杂交所得F5所衍生的143个F5:9、F5:10和F5:11重组自交系为研究材料,研究不同发育时期控制大豆籽粒干物质积累的QTL及其遗传效应对大豆籽粒干物质积累的影响。【结果】在不同发育时期检测到与大豆籽粒干物质积累相关的13个加性QTL和14对上位性QTL,其中8个加性QTL和8对上位性QTL存在与环境互作效应。另外,在本研究中仅有加性QTLdmaC2_2能够在6个发育时期都被检测到,而其它加性QTL和14对上位性QTL只能在某个或某些时期被检测到。【结论】在6个不同的发育时期,加性QTL数目、加性QTL能够解释的表型变异呈现"S"型曲线变化,与大豆籽粒干物质重的表现型变化相似,而上位性QTL能够解释的表型变异相对稳定且较小。从效应值上看,加性效应在籽粒发育开始(30d发育时期)较大,从40d发育时期开始降低,在70d发育时期降至最低,在籽粒发育结束时(80d发育时期)略有上升;上位性效应从30d发育时期到70d发育时期一直上升,在籽粒发育结束时(80d发育时期)略有下降;QTL×环境互作效应在6个发育时期均显著地影响大豆籽粒干物质的积累。从连锁群的位置上看,在6个不同的发育时期控制大豆籽粒干物质积累的加性QTL主要集中在C2连锁群(从OPK14_70到satt134区间,即QTLdmaC2_1、dmaC2_2、dmaC2_3所对应的区间),特别是发育初期(30d发育时期);从40d发育时期到籽粒发育结束时(80d发育时期),控制大豆籽粒干物质积累的加性QTL的连锁群位置变化较多,表现为发育时期的选择性。在6个不同的发育时期中,除50d发育时期以外,控制大豆籽粒干物质积累的上位性QTL主要集中在C2连锁群(从OPK14_70到satt202区间,即QTLdmaC2_1所对应的区间)和D1b连锁群(从satt537到sat_135区间,即QTLdmaD1b_1所对应的区间)之间。
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| 关 键 词： | 大豆（Glycine max L. Merri） 籽粒干物质积累 QTL 加性效应 上位性效应 环境互作效应 |
| 收稿时间： | 2009-09-07; |
Dynamic QTL Analysis of Dry Matter Accumulation in Soybean Seed at Different Developmental Stages |
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| HAN Ying-peng,TENG Wei-li,DU Yu-ping,SUN De-sheng,ZHANG Zhong-chen,XU Xiang-ling. Dynamic QTL Analysis of Dry Matter Accumulation in Soybean Seed at Different Developmental Stages[J]. Scientia Agricultura Sinica, 2010, 43(7): 1328-1338. DOI: 10.3864/j.issn.0578-1752.2010.07.002 |
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| Authors: | HAN Ying-peng TENG Wei-li DU Yu-ping SUN De-sheng ZHANG Zhong-chen XU Xiang-ling |
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| Affiliation: | HAN Ying-peng1,2,TENG Wei-li2,DU Yu-ping2,SUN De-sheng2,ZHANG Zhong-chen2,XU Xiang-ling1 (1Life Science , Technology College,Harbin Normal University,Harbin 150025,2 Soybean Research Institute/Key Laboratory of Soybean Biology of the Ministry of Education of China,Northeast Agricultural University,Harbin 150030) |
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| Abstract: | [Objective] To explore tbe additive (A), epistatic (AA) and quantitative trait locus (QTL) ×environment interaction effects (QE) of QTL on dry matter accumulation of soybean seed for deepen understanding the nature of the yield formation and accelerating the breeding process. [Method] The aim of the present study was to measure A, AA and QE effects of QTL on dry matter accumulation in a population of 143 F_(5:9), F_(5:10) and F_(5:11) derived recombinant inbred lines developed from the cross between the soybean cuitivars Charleston and Dongnong 594. [Result] Thirteen QTLs with A effect were detected at different developmental stages, eight of which had sigmficant additive × environment (AE) effect. Fourteen AA pairwise QTLs were found, eight of which showed significant epistatise × environment (AAE) effect. Furthermore, only dmaC2_2, QTL with A effect could be found from 30 day to 80 day developmental stage, other QTLs with A effect and 14 AA pairwise QTLs were only found at some developmental stages. [Conclusion] The number of QTL with A effect and phenotypic variation explained by QTL with A effect, present "S" curve from 30 day to 80 day developmental stage, which was similar to phenotypic variation of dry matter accumulation of soybean seed. However, phenotypic variation explained by these AA pairwise QTLs was relatively stable and small. Additive effect, being high in 30 day developmental stage, decreased from 40 day to 70 day developmental stage, rising at 80 day developmental stage. Epistatic effects continuously rised from 30 day to 70 day developmental stage, slightly declined at 80 day developmental stage. QTL×environment effect had a greater impact on the dry matter accumulation of soybean seed from 30 day to 80 day developmental stage. QTL with additive effect, mostly located in linkage group C2 (the interval from OPK14_70 to satt134, corresponding to QTL including dmaC2_1, dmaC2_2 and dmaC2_3) at six developmental stages, especially at 30 day developmental stage. Loci of linkage group of QTL with additive effect had most change from 40 day to maturity, which had selectivity at developmental stage. QTL with epistasis effect, mostly located between linkage group C2 (the interval from OPK14_70 to satt134, corresponding to QTL dmaC2_1) and linkage group Dlb (the interval from satt537 to sat_135, corresponding to QTL dmaD1b_1) at most developmental stages, except for 50 day developmental stage. |
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| Keywords: | QTL soybean (Glycine max L. Merri) dry matter accumulation of seed QTL,additive effect epistemic effect environment interaction effects |
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