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| 高效液相色谱法测定蜂花粉中的腺苷 | |
| 引用本文： | 岳兵,薛晓锋,吴黎明,李熠,赵静. 高效液相色谱法测定蜂花粉中的腺苷[J]. 中国农业科技导报, 2009, 11(Z1): 72-74 |
| 作者姓名： | 岳兵 薛晓锋 吴黎明 李熠 赵静 |
| 作者单位： | (中国农业科学院蜜蜂研究所, 农业部蜂产品质量监督检验测试中心, 国家农产品加工技术研发中心蜂业分中心, 北京 100093) |
| 基金项目： | 国家蜜蜂产业技术体系建设专项经费，中央级公益性科研院所基本科研业务费专项 |
| 摘 要： | 建立了一种测定蜂花粉中腺苷含量的高效液相色谱法。样品经10%乙醇提取,过 Waters OASIS柱固相萃取净化,液相色谱分离,257 nm波长处检测,腺苷在1~100 μg/mL范围内呈现良好的线性关系,R2=0.999 9,回收率较高,达到97%~101%,精密度好,相对标准偏差小于5％。该方法操作简单、准确、可靠。 |
| 关 键 词： | 蜂花粉 腺苷 固相萃取 高效液相色谱, |
| 收稿时间： | 2009-08-15 |
| 修稿时间： | 2009-10-20 |
Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography |
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| YUE Bing,XUE Xiao-feng,WU Li-ming,LI Yi,ZHAO Jing. Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography[J]. Journal of Agricultural Science and Technology, 2009, 11(Z1): 72-74 | |
| Authors: | YUE Bing XUE Xiao-feng WU Li-ming LI Yi ZHAO Jing |
| Affiliation: | (Institute of Apicultural Research, Chinese Academy of Agricultural Sciences|Bee Products Quality Supervision and Testing Center, Ministry of Agriculture|Apicultural Branch Center, Research and Development Center of National Agro-food Processing Technology, Beijing 100093, China) |
| Abstract: | A high performance liquid chromatography (HPLC) method to determine adenosine in bee pollen was established. The adenosine in the sample was extracted by 10% ethanol, purified by Waters OASIS SPE cartridge and separated with reverse phase HPLC column and detected at 257 nm. The results show that the linearity is good (R2=0.999 9), the recovery is high(97%~101%) and precision is ideal. In conclusion, this method is simple, accurate and reliable. |
| Keywords: | bee pollen adenosine SPE HPLC |
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