| 利用流式细胞术鉴定茉莉花基因组大小和染色体倍性 |
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| 引用本文： | 李春牛,李先民,黄展文,卢家仕,李琴,黄昌艳,卜朝阳. 利用流式细胞术鉴定茉莉花基因组大小和染色体倍性[J]. 热带作物学报, 2021, 42(5): 1231-1236. DOI: 10.3969/j.issn.1000-2561.2021.05.005 |
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| 作者姓名： | 李春牛 李先民 黄展文 卢家仕 李琴 黄昌艳 卜朝阳 |
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| 作者单位： | 广西农业科学院花卉研究所,广西南宁 530007;广西农业科学院农业资源与环境研究所,广西南宁 530007 |
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| 基金项目： | 广西自然科学基金项目(2020GXNSFAA297190);广西科技基地和人才专项(桂科AD17195065);广西重点研发计划项目(桂科AB18221064) |
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| 摘 要： | 以收集的16份茉莉花资源及50份实生种质为材料,以玉米B73为内参,采用流式细胞术估测茉莉花基因组大小,并以二倍体品种为对照,计算茉莉花染色体倍性。结果表明:内参与待测样品峰值能完全分开,无重叠峰,峰型清晰集中,可对茉莉花基因组大小进行有效估测;供试材料中有56份二倍体,基因组大小为0.54～0.63 Gb,有7份三倍体,基因组大小为0.79～0.96 Gb,有3份四倍体,基因组大小为1.04～1.12 Gb;从收集的资源中鉴定出二倍体和三倍体,未见四倍体,而从实生种质中鉴定出3个四倍体、2个三倍体,表明实生选种是茉莉花种质创新的一条有效途径;在已明确花冠类型的材料中,三倍体及四倍体均为单瓣型茉莉。该研究结果为茉莉花倍性育种及实生选种提供科学依据。
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| 关 键 词： | 茉莉花 流式细胞术 基因组大小 染色体倍性 实生选种 |
| 收稿时间： | 2020-06-10 |
Genome Size Estimation and Ploidy Identification of Jasminum sambac by Flow Cytometry |
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| LI Chunniu,LI Xianmin,HUANG Zhanwen,LU Jiashi,LI Qin,HUANG Changyan,BU Zhaoyang. Genome Size Estimation and Ploidy Identification of Jasminum sambac by Flow Cytometry[J]. Chinese Journal of Tropical Crops, 2021, 42(5): 1231-1236. DOI: 10.3969/j.issn.1000-2561.2021.05.005 |
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| Authors: | LI Chunniu LI Xianmin HUANG Zhanwen LU Jiashi LI Qin HUANG Changyan BU Zhaoyang |
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| Affiliation: | 1. Flowers Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China2. Agricultural Resources and Environmental Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China |
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| Abstract: | Using Zea mays ‘B73’ as the internal reference, the genome size of 66 Jasminum sambac germplasm (16 collected germplasms and 50 seedings) were estimated by the flow cytometry, and the ploidy was calculated using diploid as the control. Using Zea mays ‘B73’ as the internal parameter could effectively estimate the genome size of J. sambac, the peaks of the samples under test could be separated completely, and no overlapping peaks and peaks could be clearly concentrated. 56 germplasms were diploid with genome size between 0.54 Gb and 0.63 Gb, Seven germplasms were triploid with genome size between 0.79 Gb and 0.96 Gb, Three germplasms were tetraploid with genome size range from 1.04 Gb to 1.12 Gb. Three tetraploid and two triploid were found in the seedings, which showing that seed selection is an effective way of germplasm innovation for J. sambac. All the tetraploid and triploid blossomed were simple flower. The results of the study wouldl provide important reference basis for the ploidy breeding and seed selection of J. sambac. |
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| Keywords: | Jasminum sambac flow cytometry genome size ploidy seedling selection |
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