Fingerprinting 146 Chinese chestnut(Castanea mollissima Blume) accessions and selecting a core collection using SSR markers |
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| Authors: | Xing-hua NIE Ze-hua WANG Ning-wei LIU Li SONG Bo-qian YAN Yu XING Qing ZHANG Ke-feng FANG Yong-lian ZHAO Xin CHEN Guang-peng WANG Ling QIN Qing-qin CAO |
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| Affiliation: | 1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, P.R.China;2. College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, P.R.China;3. College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, P.R.China;4. Chestnut Technology Experiment and Extension Station of Huairou District, Beijing 102206, P.R.China;5. Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Tai''an 271000, P.R.China;6. Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli 066600, P.R.China |
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| Abstract: | Chinese chestnut is an important nut tree around the world. Although the types of Chinese chestnut resources are abundant, resource utilization and protection of chestnut accessions are still very limited. Here, we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut accessions. Our analyses showed that these markers from the tested accessions are highly polymorphic, with an average allele number(N_a) and polymorphic information content(PIC) of 8.100 and 0.622 per locus, respectively. Using these strongly distinguishing markers, we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different accessions. Our exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups: group I(North China(NC) and Northwest China(NWC) cultivar groups), group II(middle and lower reaches of the Yangtze River(MLY) cultivar group) and group III(Southeast China(SEC) and Southwest China(SWC) cultivar groups). Finally, we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut accessions. This study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups, which can serve as the basis for efficient breeding in the future. |
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| Keywords: | SSR markers fingerprinting phylogeny core collections |
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