| 抑制杨树烂皮病高效菌株的筛选及分类地位验证 |
| |
| 引用本文： | 李冲伟,宋瑞清. 抑制杨树烂皮病高效菌株的筛选及分类地位验证[J]. 中国农学通报, 2015, 31(1): 150-155. DOI: 10.11924/j.issn.1000-6850.2014-2078 |
| |
| 作者姓名： | 李冲伟 宋瑞清 |
| |
| 作者单位： | (;1.东北林业大学林学院,哈尔滨 150040；;2.黑龙江大学生命科学学院微生物学高校重点实验室,哈尔滨 150080） |
| |
| 基金项目： | 基金项目：国家林业局948引进项目“木霉菌株的引进”(2009-4-29)；哈尔滨市科技创新人才研究专项基金“电导法实现酒精发酵过程的在线监测”(RC2012QN002095)。 |
| |
| 摘 要： | 为了寻找对杨树烂皮病病原菌具有高效抑制作用的木霉菌株，采用对峙培养法和生长速率法对国内外29个木霉菌株进行了筛选，并对筛选出的高效菌株的生理生态特性和分类地位进行了研究。结果表明：菌株T-33发酵液正丁醇提取物对病原菌菌丝生长的抑制率最高，达到94.2%，对孢子萌发抑制率达到100%。菌株T-33生长最适碳源为葡萄糖，最佳用量为3%(g/L)；最适氮源为黄豆粉，最佳用量为0.02%(g/L)；培养基最适初始pH 6；最适培养温度25℃。通过18S rDNA测序分析，ITS序列长度是 583 bp，菌株T-33鉴定为Trichoderma viride。
|
| 关 键 词： | 祖代种猪场 祖代种猪场 |
| 收稿时间： | 2014-07-30 |
| 修稿时间： | 2015-01-08 |
The Screening and Physiology Characteristic of Efficient Antifungal Strain on Cytospora chrysosperma |
| |
| Li Chongwei and Song Ruiqing. The Screening and Physiology Characteristic of Efficient Antifungal Strain on Cytospora chrysosperma[J]. Chinese Agricultural Science Bulletin, 2015, 31(1): 150-155. DOI: 10.11924/j.issn.1000-6850.2014-2078 |
| |
| Authors: | Li Chongwei and Song Ruiqing |
| |
| Affiliation: | (;1.College of Forestry, Northeast Forestry University, Harbin 150040;2.Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080) |
| |
| Abstract: | In order to look for the Trichderma strains which have inhibiting effects on Chtospora chrysosperma, 29 domestic and foreign strains were screened by methods of antagonistic culture and growth-inhibiting. The morphological characteristics and physiological characteristics and taxonomic status of efficient antifungal strain were studied. The results showed that the growth-inhibiting rate of the N-butanol extract extracted from the fermented liquid of strain T-33 was 94%, and inhibition rate of spore germination was 100%. The optimal C-source of strain T-33 was glucose and the optimal content was 3% (g/L). The optimal N-source of strain T-33 was soy flour and the optimal content was 0.02% (g/L). The optimal initial pH of culture medium was 6. The optimal culture temperature was 25℃. Through 18S rDNA sequencing and phylogenies analysis of T-33, the length of ITS sequence was 583 bp, strain T-33 was verified as Trichoderma viride. |
| |
| Keywords: | Trichoderma screening Cytospora chrysosperma molecular identification |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
|
点击此处可从《中国农学通报》下载全文 |
