| 山羊酪氨酸相关蛋白1(TYRP1)基因密码子偏好性分析 |
| |
| 引用本文： | 周芳廷, 保志鹏, 赵学成, 等. 牛科物种FSHβ基因密码子偏好性分析[J]. 云南农业大学学报（自然科学）, 2018, 33(1): 47-55. DOI: 10.12101/j.issn.1004-390X(n).201702009 |
| |
| 作者姓名： | 周芳廷 保志鹏 赵学成 李卫真 杜平 康定富 董书承 余选富 苗永旺 |
| |
| 作者单位： | 1.云南农业大学 动物科学技术学院，云南 昆明 650201;2.云南农业职业技术学院 畜牧兽医学院，云南 昆明 650212;3.云南省大理州家畜繁育指导站，云南 大理 671000;4.腾冲市巴福乐槟榔江水牛良种繁育有限公司，云南 腾冲 679118 |
| |
| 摘 要： | ttttt目的ttttt为了确定牛科物种FSHβ基因密码子使用上的偏好性及差异。tttttttttttttttttt方法ttttt采用PCR产物直接测序法对36头槟榔江水牛、32头滇东南水牛、26头大额牛和29头中甸牦牛的FSHβ基因编码区序列进行了群体变异检测，并结合已发表的普通牛、野牛、山羊和绵羊的同源序列，对牛科物种FSHβ基因同义密码子使用的偏好性及其差异进行了深入分析。tttttttttttttttttt结果ttttt各牛科物种共同偏好使用UUC、CUG、AUC、GUG、UCC、AGC、CCC、CCA、ACC、ACG、GCA、UAC、CAG、ACC、GAC、GAG、UGC、AGA、AGG、GGC等20种密码子。除CCA、GCA和AGA之外，其余密码子均以G/C结尾。编码His的两个同义密码子CAU、CAC在沼泽型水牛中无偏好性，在河流型水牛中则偏好使用CAU。编码Lys的两个密码子AAA、AAG在河流型和沼泽型水牛中无偏好性，在野牛和山羊中偏好使用AAG，而牦牛、普通牛、大额牛和绵羊则偏好使用AAA。基于密码子的RSCU值构建的聚类树显示：河流型与沼泽型水牛聚为一类，牦牛、普通牛、大额牛和绵羊聚为一类，野牛与山羊聚为一类。ttttttttttttt结论ttttt各牛科物种FSHβ基因在密码子使用上均存在一定的偏好性，但差异较小，且都偏好性使用以G/C结尾的密码子，其中河流型与沼泽型水牛的偏好性最相似。ttttttttt
|
| 关 键 词： | 牛科物种 槟榔江水牛 FSHβ基因 密码子偏好性 RSCU值 聚类分析 |
| 收稿时间： | 2017-02-07 |
| 修稿时间： | 2017-05-15 |
Synonymous codon usage bias in plant mitochondrial genes is associated with intron number and mirrors species evolution |
| |
| Fangting ZHOU, Zhipeng BAO, Xuecheng ZHAO, et al. Codon Usage Bias Analysis of FSHβ Gene among Bovine Species[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(1): 47-55. DOI: 10.12101/j.issn.1004-390X(n).201702009 |
| |
| Authors: | Fangting ZHOU Zhipeng BAO Xuecheng ZHAO Weizhen LI Ping DU Dingfu KANG Shucheng DONG Xuanfu YU Yongwang MIAO |
| |
| Affiliation: | 1.Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2.Department of Animal Husbandry & Veterinary Medicine, Yunnan Agricultural College of Vocational Education, Kunming 650212, China;3.Livestock Breeding Guidance Station of Dali Prefecture, Dali 671000, China;4.BaFuLe Binglangjiang Buffalo Stock-breeding Company of Tengchong, Tengchong 679118, China |
| |
| Abstract: | tttttPurposetttttIn order to know codon usage bias of FSHβ gene and its differences among bovine species.ttttttttttttttttttMethodtttttPolymorphisms in the coding region of FSHβ gene in 36 Binglangjiang buffalo, 32 Diandongnan buffalo, 26 gayal and 29 Zhongdian yak were detected by using the method of PCR direct sequencing. Combined with published homologous sequences of cattle, bison, goat and sheep together, the data obtained in this study was analyzed to make clear the synonymous codon usage bias of FSHβ gene and its differences among bovine species.ttttttttttttttttttResulttttttThere were 20 shared preferred codons among bovine species, viz. codon UUC, CUG, AUC, GUG, UCC, AGC, CCC, CCA, ACC, ACG, GCA, UAC, CAG, ACC, GAC, GAG, UGC, AGA, AGG and codon GGC. Except for codon CCA, GCA and AGA, other codons ended with G/C. Codon CAU and CAC which encode His were different in two types of buffalo: codon CAU was preferred in river buffalo, whereas they had no usage bias in swamp buffalo. Codon AAA and AAG which encode Lys had no usage bias in river and swamp buffalo, but codon AAG was preferred in bison and goat, while codon AAA was preferred in cattle, gayal, yak and sheep. Clustering analysis based on RSCU values revealed that river and swamp buffalo clustered together, and cattle, gayal, yak and sheep clustered together, and bison and goat clustered together.tttttttttttttConclusiontttttThere are some synonymous codon usage bias among the FSHβ gene of different bovine species, but the difference was small. Furthermore, river and swamp buffalo was most similar. All bovine species prefer codons ended with G/C.ttttttttt |
| |
| Keywords: | bovine species Binglangjiang buffalo FSHβ gene codon usage bias RSCU cluster analysis |
|
| 点击此处可从《云南农业大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《云南农业大学学报(自然科学版)》下载全文 |
|
