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广西大苗山区65个茶树新品(株)系主要品质化学成分分析
引用本文:韦圻,吴河饶,陈美丽,葛智文,王熙富,杨雪梅,廖寅平,陆舒婕,陈志平,陈涛林. 广西大苗山区65个茶树新品(株)系主要品质化学成分分析[J]. 南方农业学报, 2022, 53(11): 3037-3048. DOI: 10.3969/j.issn.2095-1191.2022.11.004
作者姓名:韦圻  吴河饶  陈美丽  葛智文  王熙富  杨雪梅  廖寅平  陆舒婕  陈志平  陈涛林
作者单位:1. 贵州大学茶学院, 贵州贵阳 550025;2. 柳州市绿化建设发展中心, 广西柳州 545001;3. 柳州市农业技术推广中心, 广西柳州 545003;4. 柳州市林业科学研究所, 广西柳州 545300;5. 融水县农业农村局, 广西柳州 545300
基金项目:国家自然科学基金项目(32260787)贵州大学人才引进项目(贵大人基合字[2019]25号)贵州省科技支撑计划项目(黔科合支撑[2021]一般188)
摘    要:【目的】分析广西大苗山区65个茶树新品(株)系的品质化学成分特性和适制性,为培育具有地方特色的茶树新品种提供理论参考。【方法】以广西大苗山区地方茶树种质资源筛选培育出的65个茶树新品(株)系为研究对象,采用常规生化分析方法和高效液相色谱法对其主要品质化学成分进行测定,计算Shannon-Weaver遗传多样性指数(H'),并对其进行主成分分析、聚类分析、相关分析和适制性分析,最后筛选出具有优良种质潜力的茶树品(株)系。【结果】65个茶树新品(株)系的遗传多样性指数为1.45~2.07,平均为1.88,变异系数为17.37%~97.70%,平均为36.75%,表现出丰富的遗传变异。基于品质化学成分测定结果对65个茶树新品(株)进行聚类分析,在遗传距离14.5处,可将分为六大类群,第Ⅰ、Ⅱ、Ⅲ和Ⅴ类群包含45、9、7和2个品(株)系,第Ⅳ类群和Ⅵ类群分别包含1个品(株)系。对65个茶树品(株)系的15个品质化学成分及指标进行相关分析,结果显示,呈显著正相关的有12对,呈显著负相关的有5对,呈极显著正相关的有34对,呈极显著负相关的有14对。对15个化学成分及指标进行主成分分析,提取了4个主成分,累计贡献率达80.80%。在65个茶树新品(株)系中,适制绿茶的茶树新品(株)系有55个,红绿兼制的有10个,其中具有优良种质潜力的茶树新品(株)系共有16个。【结论】广西大苗山区65个茶树新品(株)系的品质化学成分多样性丰富,存在明显遗传变异,具有培育优良茶树新品种的潜力。

关 键 词:茶树新品(株)系   品质化学成分   遗传多样性   适制性   广西大苗山区
收稿时间:2022-06-23

Main quality chemical components of 65 new tea plant strains in Damiao Mountain of Guangxi
WEI Qi,WU He-rao,CHEN Mei-li,GE Zhi-wen,WANG Xi-fu,YANG Xue-mei,LIAO Yin-ping,LU Shu-jie,CHEN Zhi-ping,CHEN Tao-lin. Main quality chemical components of 65 new tea plant strains in Damiao Mountain of Guangxi[J]. Journal of Southern Agriculture, 2022, 53(11): 3037-3048. DOI: 10.3969/j.issn.2095-1191.2022.11.004
Authors:WEI Qi  WU He-rao  CHEN Mei-li  GE Zhi-wen  WANG Xi-fu  YANG Xue-mei  LIAO Yin-ping  LU Shu-jie  CHEN Zhi-ping  CHEN Tao-lin
Affiliation:1. Tea College, Guizhou University, Guiyang, Guizhou 550025, China;2. Liuzhou Greening Construction Development Center, Liuzhou, Guangxi 545001, China;3. Liuzhou Agricultural Technology Extension Center, Liuzhou, Guangxi 545003, China;4. Liuzhou Forestry Science Research Institute, Liuzhou, Guangxi 545300, China;5. Rongshui County Agricultural and Rural Bureau, Liuzhou, Guangxi 545300, China
Abstract:【Objective】To identify 65 new tea plant strains in the Damiao Mountain area of Guangxi,analyze their quality chemical composition characteristics and suitability,and lay the scientific foundation for cultivating new tea plant varieties with local characteristics.【Method】The 65 new tea plant strains bred by germplasm resources in Damiao Mountain of Guangxi were as objects. The main quality chemical components were determined and analyzed by conventional biochemical analysis and high performance liquid chromatography(HPLC) ;their Shannon-Weaver diversity index(H') was analyzed by statistical software,and principal component analysis,cluster analysis,correlation analysis and suitability analysis were conducted. Finally,the tea plant strains with excellent germplasm resource potential were screened.【Result】The diversity indexes of 65 new tea plant strains ranged from 1.45 to 2.07,with an average of 1.88,and the coefficients of variation ranged from 17.37% to 97.70%,with an average of 36.75%,showing a rich genetic variation. The 65 new tea plant strains were clustered based on quality chemical components determination,and at a genetic distance of 14.5,they could be divided into six taxa,with taxon Ⅰ containing 45 strains,taxon Ⅱ containing 9 strains,taxon Ⅲ containing 7 strains,taxon Ⅳ containing 1 strain,taxon Ⅴ containing 2 strains,taxon Ⅳ containing 1 and taxon Ⅵ containing 1 strain.The correlation analysis of the 15 chemical components and indexes of the 65 new tea plant strains showed that there were 12 pairs of significant positive correlations,5 pairs of significant negative correlations,34 pairs of highly significant positive correlations and 14 pairs of highly significant negative correlations. Principal component analysis was performed on the 15 chemical components and indexes,and 4 principal components were extracted with a cumulative contribution of variance of 80.80%. Among the 65 new tea plant strains,55 new tea plant strains were suitable for green tea production,and 10 were suitable for both red tea and green tea,and 16 were germplasm resources with excellent germplasm potential.【Conclusion】The 65 new tea plant strains in the Damiao Mountain area of Guangxi have rich diversity of quality chemical components and obvious genetic variation,and have the potential to cultivate excellent new tea plant varieties.
Keywords:
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