| 多粘类芽胞杆菌分离鉴定及其对甘薯蔓割病的防治效果 |
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| 引用本文： | 徐颖华,柴沙沙,李成阳,张文英,王崇,雷剑,靳晓杰,宋峥,杨新笋,王连军. 多粘类芽胞杆菌分离鉴定及其对甘薯蔓割病的防治效果[J]. 南方农业学报, 2022, 53(4): 1057-1065. DOI: 10.3969/j.issn.2095-1191.2022.04.019 |
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| 作者姓名： | 徐颖华 柴沙沙 李成阳 张文英 王崇 雷剑 靳晓杰 宋峥 杨新笋 王连军 |
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| 作者单位： | 1 湖北省农业科学院粮食作物研究所/湖北省甘薯工程技术研究中心/粮食作物种质创新与遗传改良湖北省重点实验室, 湖北武汉 430064;2 长江大学农学院, 湖北荆州 434025 |
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| 基金项目： | 国家重点研发计划项目(2019YFD1001300,2019YFD1001305)湖北省重点研发计划项目(2020BHB024)国家现代农业产业技术体系建设专项(CARS-10) |
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| 摘 要： | 【目的】筛选对甘薯蔓割病病原菌具有拮抗作用的芽胞杆菌,为甘薯蔓割病的生物防治提供菌种资源。【方法】通过平板对峙法从玉米根际土壤中筛选对甘薯蔓割病病原菌具有拮抗作用的芽胞杆菌;通过生物学特征和16S rDNA序列分析对拮抗菌株进行分类鉴定;通过盆栽试验进行拮抗菌株对甘薯蔓割病的防效试验,并检测拮抗菌株对甘薯叶片脱落酸(ABA)和赤霉素(GA)含量,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性及可溶性蛋白含量的影响。【结果】从玉米根际土壤中分离获得1株乳白色且具有拮抗作用的芽胞杆菌,命名为HAAS05,通过16S rDNA鉴定及系统发育进化树比对分析,将HAAS05菌株鉴定为多粘类芽胞杆菌(Paenibacillus polymyxa)。盆栽试验结果显示,HAAS05菌株对甘薯蔓割病有明显的拮抗效果;OD600 nm=0.20和OD600 nm=0.40的HAAS05菌悬液处理第10 d后,甘薯叶片中ABA含量较仅接种病原菌处理(201.94 ng/g)显著降低(P<0.05,下同),分别为52.29和107.10 ng/g,而GA含量较仅接种病原菌处理(7.05 ng/g)显著升高,分别为8.10和10.31 ng/g,甘薯叶片SOD活性和可溶性蛋白含量均较仅接种病原菌处理显著升高,而POD活性变化无明显规律。【结论】多粘类芽胞杆菌HAAS05菌株对甘薯蔓割病有较好的拮抗作用,具有一定的开发应用潜力。
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| 关 键 词： | 甘薯 蔓割病 多粘类芽胞杆菌 生物防治 |
| 收稿时间： | 2021-10-22 |
Isolation and identification of Paenibacillus polymyxa strain and its control effect on Fusarium bulbigenum of sweet potato |
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| XU Ying-hua,CHAI Sha-sha,LI Cheng-yang,ZHANG Wen-ying,WANG Chong,LEI Jian,JIN Xiao-jie,SONG Zheng,YANG Xin-sun,WANG Lian-jun. Isolation and identification of Paenibacillus polymyxa strain and its control effect on Fusarium bulbigenum of sweet potato[J]. Journal of Southern Agriculture, 2022, 53(4): 1057-1065. DOI: 10.3969/j.issn.2095-1191.2022.04.019 |
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| Authors: | XU Ying-hua CHAI Sha-sha LI Cheng-yang ZHANG Wen-ying WANG Chong LEI Jian JIN Xiao-jie SONG Zheng YANG Xin-sun WANG Lian-jun |
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| Affiliation: | 1 Food Crops Institute, Hubei Academy of Agricultural Sciences/Hubei Sweet Potato Engineering Technology Research Center/Hubei Provincial Key Laboratory of Food Crop Germplasm Innovation and Genetic Improvement, Wuhan, Hubei 430064, China;2 College of Agriculture, Yangtze University, Jingzhou,Hubei 434025, China |
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| Abstract: | 【Objective】To screen Bacillus with antagonistic effect on Fusarium bulbigenum and provide strain resources for biological control of F. bulbigenum.【Method】Bacillus antagonistic to F. bulbigenum was screened from maize rhizosphere soil by plate antagonism method;classification and identification by biological characteristics and 16S rDNA sequence analysis;the control effect of isolated strains against F. bulbigenum was tested by pot experiment,and the effects of isolated strains on the contents of abscisic acid(ABA)and gibberellin(GA),the activities of superoxide dismutase(SOD) and peroxidase(POD) and soluble protein of sweet potato leaves were detected.【Result】A milky white strain with antagonistic effect was isolated from the rhizosphere soil of maize,named as HAAS05.Through 16S rDNA sequence analysis and phylogenetic tree analysis,HAAS05 strain was identified as Paenibacillus polymyxa.The biocontrol experiment of strain HAAS05 was carried out by pot experiment,and the hormone content,enzyme activity and soluble protein content of the tested plants were detected.The results of pot experiment showed that HAAS05 strain had obvious antagonistic effect on F. bulbigenum.After 10 d treatment with HAAS05 bacterial suspensions with OD600 nm=0.20and OD600 nm=0.40,the ABA content in sweet potato leaves was significantly lower than that in the treatment only inoculated with pathogens(201.94 ng/g)(P<0.05,the same below),which were 52.29 and 107.10 ng/g,respectively.The GA content was significantly higher than that in the treatment that only inoculated with pathogens(7.05 ng/g),which were8.10 and 10.31 ng/g,respectively.The SOD activity and soluble protein content in sweet potato leaves were significantly higher than those in the treatment only inoculated with pathogens.There was no significant change in the content of POD enzyme in the plant.【Conclusion】P.polymyxa HAAS05 has good biocontrol effect on F. bulbigenum and has certain application and development potential. |
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