| 小尾寒羊泌乳性状重要lncRNAs的筛选、鉴定及功能分析 |
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| 引用本文： | 王继卿,郝志云,沈继源,柯娜,黄兆春,梁维炜,罗玉柱,胡江,刘秀,李少斌. 小尾寒羊泌乳性状重要lncRNAs的筛选、鉴定及功能分析[J]. 中国农业科学, 2021, 54(14): 3113-3123. DOI: 10.3864/j.issn.0578-1752.2021.14.016 |
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| 作者姓名： | 王继卿 郝志云 沈继源 柯娜 黄兆春 梁维炜 罗玉柱 胡江 刘秀 李少斌 |
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| 作者单位： | 甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室/甘肃省牛羊基因改良工程实验室,兰州 730070 |
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| 基金项目： | 国家自然科学基金(32060746);国家自然科学基金(31860635);甘肃农业大学“伏羲青年英才培育计划(Gaufx-02Y02);甘肃省基础研究创新群体项目(18JR3RA190);甘肃农业大学自列课题(GSAU-ZL-2015-033) |
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| 摘 要： | [目的]长链非编码RNA(long non-coding RNAs,lncRNAs)是一类长度大于200 nt的非编码RNA分子,它对奶牛和奶山羊的乳腺发育和泌乳过程发挥了重要调控作用,然而在绵羊上研究甚少.为此开展lncRNAs对绵羊泌乳性能的调控作用研究,为解析绵羊泌乳性能的分子机理提供理论基础.[方法]选取高泌乳...
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| 关 键 词： | 绵羊 乳腺 长链非编码RNA(lncRNA) 泌乳期 |
| 收稿时间： | 2020-11-13 |
Screening,Identification and Functional Analysis of Important LncRNAs for Lactation Traits in Small-Tailed Han Sheep |
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| WANG JiQing,HAO ZhiYun,SHEN JiYuan,KE Na,HUANG ZhaoChun,LIANG WeiWei,LUO YuZhu,HU Jiang,LIU Xiu,LI ShaoBin. Screening,Identification and Functional Analysis of Important LncRNAs for Lactation Traits in Small-Tailed Han Sheep[J]. Scientia Agricultura Sinica, 2021, 54(14): 3113-3123. DOI: 10.3864/j.issn.0578-1752.2021.14.016 |
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| Authors: | WANG JiQing HAO ZhiYun SHEN JiYuan KE Na HUANG ZhaoChun LIANG WeiWei LUO YuZhu HU Jiang LIU Xiu LI ShaoBin |
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| Affiliation: | College of Animal Science and Technology/Gansu Key Laboratory of Herbivorous Animal Biotechnology/Gansu Engineering Lab of Genetic Improvement in Ruminants, Gansu Agricultural University, Lanzhou 730070 |
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| Abstract: | 【Objective】Long non-coding RNAs (lncRNAs) are a type of non-coding RNAs with >200 nt in length, which have been shown to regulate mammary gland development and lactation process in dairy cows and dairy goats. However, little is known about the effect of lncRNAs on milk traits in sheep. The aim of the study was to analyze the effect of lncRNAs on milk performance and then provided a theoretical basis for elucidating molecular mechanism of lactation performance in sheep.【Method】Three high-lactating yield and high-milk-fat-content Small-Tailed Han sheep and three low-lactating yield and low-milk-fat-content Small-Tailed Han sheep were selected to profile the expression of lncRNAs in the mammary gland tissues during lactation using RNA-Seq. The enrichment analysis was performed using GO and KEGG databases for the target genes of differentially expressed lncRNAs between the two groups. The expression levels of 16 differentially expressed lncRNAs were verified using reverse transcription-quantitative PCR (RT-qPCR).【Result】A total of 7 239 expressed lncRNAs were identified in the mammary gland tissues of Small-Tailed Han sheep, including 2 262 known lncRNAs and 4 977 novel lncRNAs. The most of lncRNAs were expressed at low levels. The 120 differentially expressed lncRNAs were found between the two groups of Small-Tailed Han sheep, of which 68 lncRNAs were up-regulated in high-lactating performance Small-Tailed Han sheep, while 52 lncRNAs were down-regulated. The target genes of differentially expressed lncRNAs were significantly enriched in sulfur compound metabolic process, thioester biosynthesis process, acyl-CoA biosynthetic process, Rap1 signal pathway and adhesion junction. The lncRNA- miRNA network showed that some target miRNAs of the six most differentially expressed lncRNAs including MSTRG.125242.6 and MSTRG.59580.8, play important roles in mammary gland development and lactation in domestic animals. The RT-qPCR results showed that the expression tendency of 16 lncRNAs was consistent with the RNA-Seq results, which confirmed the accuracy and authenticity of the RNA-Seq data.【Conclusion】The differentially expressed lncRNAs screened were involved in the regulation of mammary gland development and milk performance in sheep and the results will provide a theoretical basis for analyzing the molecular genetic mechanism of lactation performance in sheep. |
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| Keywords: | sheep mammary gland long non-coding RNA (lncRNA) lactation period |
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