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AFLP引物组合数对准确分析马缨杜鹃遗传多样性的影响
引用本文:沈德周, 纵丹, 周安佩, 颜璐茜, 李旦, 何承忠. AFLP引物组合数对准确分析马缨杜鹃遗传多样性的影响[J]. 西南林业大学学报, 2016, 36(6): 22-28.doi:10.11929/j.issn.2095-1914.2016.06.004
作者姓名:沈德周  纵丹  周安佩  颜璐茜  李旦  何承忠
作者单位:1. 西南林业大学云南省高校林木遗传改良与繁育重点实验室,云南 昆明 650224;; 2. 西南林业大学西南地区生物多样性保育国家林业局重点实验室,云南 昆明 650224; ;3. 西南林业大学云南生物多样性研究院,云南 昆明 650224;; 4. 西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,云南 昆明 650224
基金项目:云南省中青年学术与技术带头人后备人才培养项目 (2012HB021) 资助;西南林业大学云南省省级重点学科 (林学) 建设项目资助;西南林业大学大学生创新基金项目 (C15070) 资助;西南林业大学云南省高校林木遗传改良与繁育重点实验室开放基金项目 (YNGB201505) 资助。
摘要:利用5、7、9、11和13对AFLP引物组合对4个居群马缨杜鹃遗传多样性进行分析,探讨不同引物组合数量对其遗传多样性参数指标的影响。结果表明:随着引物组合数量的增加,紫溪山居群遗传多样性的各参数指标变化趋势一致, 5对引物组合检测结果略高于其他引物组合数,但差异不显著,其余3个居群的变化规律不明显;不同引物组合数量对居群间遗传距离无显著影响,UPGMA聚类结果完全相同。由此可见,选用5对引物组合对马缨杜鹃进行遗传多样性分析就能够提供较为准确的结果。
摘    要:利用5、7、9、11和13对AFLP引物组合对4个居群马缨杜鹃遗传多样性进行分析,探讨不同引物组合数量对其遗传多样性参数指标的影响。结果表明:随着引物组合数量的增加,紫溪山居群遗传多样性的各参数指标变化趋势一致, 5对引物组合检测结果略高于其他引物组合数,但差异不显著,其余3个居群的变化规律不明显;不同引物组合数量对居群间遗传距离无显著影响,UPGMA聚类结果完全相同。由此可见,选用5对引物组合对马缨杜鹃进行遗传多样性分析就能够提供较为准确的结果。

关 键 词:马缨杜鹃   遗传多样性   AFLP标记   引物
收稿时间:2015-12-14

Effect of the Quantity of AFLP Primer Combinations on Accurately Analyzing Rhododendron delavayi Genetic Diversity
Shen Dezhou1, 2, Zong Dan1, 2, Zhou Anpei1 and 2. Effect of the Quantity of AFLP Primer Combinations on Accurately Analyzing Rhododendron delavayi Genetic Diversity[J]. Journal of Southwest Forestry University, 2016, 36(6): 22-28.doi:10.11929/j.issn.2095-1914.2016.06.004
Authors:Shen Dezhou1  2   Zong Dan1  2   Zhou Anpei1  2
Affiliation:1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming Yunnan 650224, China; ;2. Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming Yunnan 650224, China; ;3. Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming Yunnan 650224, China; ;4. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming Yunnan 650224, China
Abstract:In order to investigate the effect of the quantity of primer combinations on genetic diversity parameters in Rhododendron delavayi, the amplified fragment length polymorphism (AFLP) was employed to test 4 natural populations of Rhododendron delavayi with 5, 7, 9, 11 and 13 AFLP primer combinations. The results showed that the genetic diversity parameters of Zixi Mountain population had same changing trends with the increase of primer combinations quantity, which indicated that the amplified resutls with 5 primer combinations were slightly higher than that with other primer combinations, but the differences were not significant. The changing trends of genetic diversity parameters in other 3 populations were not obvious. There was no significant effect on the genetic distance between populations and the cluster results were consistent based on unweighted pair group method analysis (UPGMA) with different numbers of primer combinations. So, it could be concluded that 5 primer combinations could provide accurate results of Rdelavayi genetic diversity.
Keywords:Rhododendron delavayi   genetic diversity   AFLP markers   primer
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