| 溃疡病菌Botryosphaeria dothidea粗毒素产生的条件及特性 |
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| 引用本文： | 梁军,魏淑花,叶建仁,张星耀. 溃疡病菌Botryosphaeria dothidea粗毒素产生的条件及特性[J]. 浙江农林大学学报, 2008, 25(5): 559-564 |
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| 作者姓名： | 梁军 魏淑花 叶建仁 张星耀 |
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| 作者单位： | 1.国家林业局 森林生态环境与保护重点实验室，北京 100091;2.中国林业科学研究院 森林生态环境与保护研究所，北京 100091;3.宁夏回族自治区农林科学院 植物保护研究所，宁夏 银川 750002 |
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| 摘 要： | 为了研究溃疡病菌的致病机制，以葡萄座腔菌Botryosphaeria dothidea 产生的毒素对北京杨Populus × beijingensis愈合组织的褐化程度为评价指标，对溃疡病菌产生粗毒素的条件和粗毒素的基本特性进行了初步研究。结果表明，溃疡病菌在改良Fries No.3液体培养基，25 ℃温度，光照，振荡培养，21 d后所得的粗毒素毒性最强；该病原菌在偏酸性和中性条件（pH 5.60和7.00）下易于产毒；产生的粗毒素对热不稳定，经121 ℃高温，0.11 MPa压力处理15 min后，其生物活性丧失；这种粗毒素对酸碱具有一定的稳定性，pH 4.69及8.69时，粗毒素的生物活性虽有一定程度的下降，但与自然pH条件下的粗毒素的生物活性差异不显著。图5表3参18
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| 关 键 词： | 森林保护学 北京杨 溃疡病 毒素 葡萄座腔菌 愈合组织 |
Crude toxin production of Botryosphaeria dothidea:initial conditions and characteristics |
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| LIANG Jun,WEI Shu-hua,YE Jian-ren,ZHANG Xing-yao. Crude toxin production of Botryosphaeria dothidea:initial conditions and characteristics[J]. Journal of Zhejiang A&F University, 2008, 25(5): 559-564 |
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| Authors: | LIANG Jun WEI Shu-hua YE Jian-ren ZHANG Xing-yao |
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| Affiliation: | 1.Key Laboratory of Forest Ecology and Environment Protection，State Forestry Administration，Beijing 100091，China;2.Research Institute of Forest Ecology，Environment and Protection，The Chinese Academy of Forestry，Beijing 100091，China;3.Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences，Yinchuan 750002，Ningxia，China |
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| Abstract: | To study pathogenic mechanisms，initial conditions and characteristics of crude toxins produced from Botryosphaeria dothidea（Moug. ex Fr.） Ces. de Not. were first analyzed using the degree of browning on callus of Populus × beijingensis as the appraisal index. Results showed that with light，shaking，being cultured in a modified Fries No.3 liquid medium at 25 °C for 21 days，and a pH of 5.60 （acidic） and 7.00 （neutral），was the optimum condition for crude toxins production by B. dothidea. Additionally，produced more toxin. When treated with high temperature（121 °C） and high pressure（0.11 MPa） for 15 minutes，the toxins exhibited thermal instability and lost their bioactivity. However，with acidity and alkalinity they did remain active. Although toxicity decreased slightly，activity with this higher temperature and pressure was similar（P＞0.05） to toxins with pH of 4.69 and 8.69. ［Ch，5 fig. 3 tab. 18 ref.］ |
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