| 菲白竹组织培养技术 |
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| 引用本文： | 杨海芸,桂仁意,汤定钦,方伟. 菲白竹组织培养技术[J]. 浙江农林大学学报, 2008, 25(2): 255-258 |
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| 作者姓名： | 杨海芸 桂仁意 汤定钦 方伟 |
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| 作者单位： | 1.浙江林学院浙江省现代森林培育重点实验室, 浙江临安 311300 |
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| 基金项目： | 浙江省科学技术重点项目(2003C22025) |
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| 摘 要： | 以菲白竹Sasa fortunei幼嫩竹秆和竹鞭为材料,研究其组织培养快繁关键技术。结果表明:以竹鞭为外植体萌芽率较高,添加6-苄基腺嘌呤(6-BA)试管苗增殖效果优于噻二唑苯基脲(TDZ),但TDZ能促进新芽生根;以MS(Murashige and Skoog)为基本培养基,分别添加3 mg·L-1 6-BA与0.5 mg·L-1萘乙酸(NAA),侧芽增殖系数达3.60;添加3 mg·L-1 6-BA与0.01 mg·L-1 TDZ,增殖系数较高(3.43),且伸长生长迅速,生根率达到100%,可同时实现增殖与生根,有利于菲白竹大规模快速繁殖。
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| 关 键 词： | 植物学 菲白竹 组织培养 快速繁殖 |
| 收稿时间： | 2007-02-13 |
Micropropagation of Sasa fortunei |
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| YANG Hai-yun,GUI Ren-yi,TANG Ding-qin,FANG Wei. Micropropagation of Sasa fortunei[J]. Journal of Zhejiang A&F University, 2008, 25(2): 255-258 |
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| Authors: | YANG Hai-yun GUI Ren-yi TANG Ding-qin FANG Wei |
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| Affiliation: | 1.Key Laboratory for Modern Silviculture Technology of Zhejiang Province, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China |
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| Abstract: | Micropropagation of Sasa fortunei was studied with shoots of young culms and rhizomes as explants. The results showed as follows:germination rate of rhizomes was better than that of culms. 6-Benzylaminopurine(6-BA) had a better effect on proliferation than thidazuron(TDZ). But TDZ was able to improve rooting. The highest proliferation rate(3.60) was obtained from the combination of 3 mg·L-1 6-BA and 0.5 mg·L-1 NAA. The combination of 3 mg·L-1 6-BA and 0.01 mg·L-1 TDZ also had a high proliferation rate (3.43),achieving 100% rooting percentage and good growth performance. Therefore,it was potentially propitious to mass production. |
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