| 体外产气法研究不同NFC/NDF底物条件下外源纤维素酶的适宜添加水平 |
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| 引用本文： | 陈光吉,宋善丹,彭忠利,王普昶,吴佳海,王小利,郭春华,王子苑,高彦华,李小冬,柏雪,付锡三. 体外产气法研究不同NFC/NDF底物条件下外源纤维素酶的适宜添加水平[J]. 草业学报, 2017, 26(7): 116-127. DOI: 10.11686/cyxb2017055 |
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| 作者姓名： | 陈光吉 宋善丹 彭忠利 王普昶 吴佳海 王小利 郭春华 王子苑 高彦华 李小冬 柏雪 付锡三 |
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| 作者单位： | 1.西南民族大学生命科学与技术学院,四川 成都 610041; 2.贵州省草业研究所,贵州 贵阳 550006; 3.乐至县科技局,四川 乐至 641500 |
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| 基金项目： | “科技部科技富民强县专项:川中黑山羊(乐至型)健康养殖关键技术集成与示范”;科技创新人才团队建设项目“贵州省喀斯特山区草地培育与养殖管理科技创新人才团队”，黔科合重大专项字(2014)6017号资助 |
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| 摘 要： | 本试验旨在利用体外产气法研究不同非纤维性碳水化合物/中性洗涤纤维(non-fiber carbohydrates/neutral detergent fiber, NFC/NDF)的底物条件下外源纤维素酶(exogenous fibrolytic enzymes, EFE)对底物产气、降解和发酵特性的影响,找出不同底物条件下适宜的EFE添加水平。采用2×5交叉分组试验设计,5个NFC/NDF底物水平 (0.85、1.02、1.19、1.36和1.53)分别添加5种水平的EFE(0, 2, 4, 8 和16 mg/g DM)进行体外发酵。结果表明,1)不同NFC/NDF底物和EFE水平对体外总产气量(GP48)和产气参数(b、c和L)均有显著的影响(P<0.05),且两因素互作显著(P<0.05);底物1中, GP48、b和c值随EFE剂量的增加显著地线性和二次提高(P<0.05),其中以16 mg/g 水平组较高,L值则呈相反趋势;底物2~4中,GP48、b和c值随EFE剂量增加显著地二次提高(P<0.05),其中以4 mg/g 水平组较高。2)不同底物对干物质消失率(DMD)、酸性洗涤纤维降解率(ADFD)、中性洗涤纤维降解率(NDFD)和氮降解率(ND)均有显著的影响(P<0.05),除ND外,EFE水平的添加效应呈现相似结果;底物1条件下,随着EFE添加剂量的提高,DMD、ADFD和NDFD显著地线性提高(P<0.05),其中均以16 mg/g组最高(P<0.05);而底物2~4中则以4 mg/g组最高(P<0.05)。3)不同NFC/NDF底物和EFE水平对瘤胃发酵参数均有显著影响(P<0.05),除丙酸外,两种因素对其他指标的互作效应均显著(P<0.05);在底物1~4条件下,随着EFE添加剂量的提高,除丙酸以外的其他发酵参数显著地线性和二次提高或降低(P<0.05);底物1中,对照组pH值、氨态氮含量和丁酸摩尔浓度显著高于4、8和16 mg/g组(P<0.05),相反,16 mg/g组的总挥发性脂肪酸(TVFA)、乙酸摩尔浓度和乙酸∶丙酸值较高;底物2~4条件下,pH值和氨态氮含量以4 mg/g组较低,而TVFA、乙酸摩尔浓度和乙酸∶丙酸值则呈相反的趋势。4)NFC/NDF=1.53时,添加EFE对体外产气参数、降解特性和发酵特性均无显著影响(P>0.05)。由此,本试验发现,NFC/NDF底物影响了EFE的添加效应,NFC/NDF=0.85时,16 mg/g EFE水平组有较好纤维降解效果;NFC/NDF分别为1.02、1.19和1.36时,EFE最适添加剂量为4 mg/g;NFC/NDF=1.53时,底物中添加EFE没有正面效应。
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| 关 键 词： | 体外产气法 NFC/NDF 外源纤维素酶 适宜添加水平 |
| 收稿时间： | 2017-02-20 |
Determination of appropriate levels of exogenous fibrolytic enzyme supplementation for substrates with different non-fiber carbohydrate/neutral detergent fiber ratios based on in vitro gas production |
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| CHEN Guang-Ji,SONG Shan-Dan,PENG Zhong-Li,WANG Pu-Chang,WU Jia-Hai,WANG Xiao-Li,GUO Chun-Hua,WANG Zi-Yuan,GAO Yan-Hua,LI Xiao-Dong,BAI Xue,FU Xi-San. Determination of appropriate levels of exogenous fibrolytic enzyme supplementation for substrates with different non-fiber carbohydrate/neutral detergent fiber ratios based on in vitro gas production[J]. Acta Prataculturae Sinica, 2017, 26(7): 116-127. DOI: 10.11686/cyxb2017055 |
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| Authors: | CHEN Guang-Ji SONG Shan-Dan PENG Zhong-Li WANG Pu-Chang WU Jia-Hai WANG Xiao-Li GUO Chun-Hua WANG Zi-Yuan GAO Yan-Hua LI Xiao-Dong BAI Xue FU Xi-San |
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| Affiliation: | 1.College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China; 2.Guizhou Institute of Prataculture, Guiyang 550006, China; 3.Bureau of Science and Technology, Sichuan Province, Lezhi 641500, China |
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| Abstract: | The objective of this study was to determine the appropriate level of exogenous fibrolytic enzyme (EFE) supplementation for substrates with different non-fiber carbohydrates/neutral detergent fiber (NFC/NDF) ratios.The experiment had a cross group design with two factors and five levels.Five substrates with different NFC/NDF ratios (0.85,1.02,1.19,1.36,and 1.53;sub1-5) were supplemented with EFE at five levels (0,2,4,8,and 16 mg/g dry matter/DM) and in vitro gas production,degradation,and fermentation profiles were determined.The results can be summarized as follows:1) The NFC/NDF ratio influenced total in vitro gas production (GP48) and gas production parameters (b,c,and L) (P＜0.05).The interaction between NFC/NDF ratio and EFE level was significant for gas production parameters (P＜0.05).In sub1,as the EFE level increased,GP48,b,and c increased (linear and quadratic effect,P＜0.05),and the highest GP48,b,and c were in the 16 mg/g EFE treatment;L showed the opposite trend.In the sub2-4 treatments,the highest GP48,b,and c were in the 4 mg/g EFE treatment.2) The NFC/NDF ratio in the substrate significantly affected the dry matter disappearance rate (DMD),acid detergent fiber degradation rate (ADFD),neutral detergent fiber degradation rate (NDFD),and nitrogen degradation rate (ND) (P＜0.05).The additive effects of EFE produced similar results,except for ND.In sub1,as the EFE dose increased,DMD,ADFD,and NDFD linearly increased (P＜0.05).The maximum values of DMD,ADFD,and NDFD were in the 16 mg/g EFE treatment in sub1 and in the 4 mg/g EFE treatment in sub2-4 (P＜0.05).3) The fermentation profile differed significantly among substrates with different NFC/NDF ratios and different levels of EFE supplementation (P＜0.05),and the two factors had a significant interaction effect (P＜0.05) for all fermentation parameters,except for propionate.For sub1-4,as the EFE levels increased,the fermentation profiles of all parameters except for propionate increased or decreased (quadratic effect,P＜0.05).In sub1,pH,ammonia-N,and the butyrate molar concentration were higher in the control group than in the 4,8 and 16 mg/g EFE treatments,but the acetate molar concentration and acetate:propionate ratio were higher in the 16 mg/g EFE treatment.The sub2-4 treatments showed lower pH and ammonia-N,and higher total volatile fatty acid (TVFA),acetate molar concentration,and acetate:propionate ratio.4) In the substrate with NFC/NDF=1.53,EFE supplementation had no effect on in vitro gas production,fiber degradation,or fermentation characteristics (P＞0.05).The results of this study showed that the NFC/NDF ratio influenced the response to EFE supplementation;NFC/NDF=0.85,16 mg/g EFE showed the best fiber degradation,and the optimum EFE dose was 4 mg/g for substrates with NFC/NDF ratios of 1.02,1.19,and 1.36.There was no positive effect of EFE on the substrate with NFC/NDF---1.53. |
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| Keywords: | in vitro gas production NFC/NDF exogenous fibrolytic enzymes appropriate supplementary dose |
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