| 常春藤离体快繁技术 |
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| 引用本文： | 张存旭,杨锋利,袁秀平. 常春藤离体快繁技术[J]. 浙江农林大学学报, 2005, 22(2): 241-245 |
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| 作者姓名： | 张存旭 杨锋利 袁秀平 |
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| 作者单位： | 1.西北农林科技大学 林学院, 陕西 杨凌 712100;2.杨凌职业技术学院 林学系, 陕西 杨凌 712100 |
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| 基金项目： | 陕西省科技攻关项目(2001K01-G7-01) |
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| 摘 要： | 以常春藤Hedera nepalensis var .sinensis 茎段为外植体, 研究了培养基、植物生长调节物质和移栽基质对常春藤离体快繁的影响。结果表明:起始和增殖培养中高盐质量浓度的MS 培养基好于低盐培养基WPM 和B5 。增殖培养时, 在培养基中同时添加2.0 mgL-1 6-BA和1.0 mgL-1GA3 为佳, 增殖系数可达到4 。以1/2 MS 为基本培养基, 虽然不添加任何生长调节物质亦能获得高生根率, 但附加0.10 mgL-1NAA 能更好地促进根系发育。移栽基质选用腐质土, 成活率可达80 %以上。用自来水代替蒸馏水, 白砂糖代替蔗糖, 对常春藤试管苗的增殖和生长无显著影响, 可降低培养成本。表6 参9
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| 关 键 词： | 育苗 常春藤 快速繁殖 体外培养 |
| 收稿时间： | 2004-07-06 |
In vitro rapid propagation of Hedera nepalensis var .sinensis |
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| ZHANG Cun-xu,YANG Feng-li,YUAN Xiu-ping. In vitro rapid propagation of Hedera nepalensis var .sinensis[J]. Journal of Zhejiang A&F University, 2005, 22(2): 241-245 |
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| Authors: | ZHANG Cun-xu YANG Feng-li YUAN Xiu-ping |
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| Affiliation: | 1..Institute of Forestry Science , Northwest A&F University , Yangling 712100 , Shaanxi , China;2.Department of Forestry , Yangling Vocational and Technical College , Yangling 712100, Shaanxi , China |
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| Abstract: | Using nodal segments as a source of initial explants , the effects of basal medium, plant growth substances as well as transplantation medium on rapid propagation of Hedera neplensis var .sinensis was studied . The results showed that the rich salt medium MS is more suitable for initial and multiplication culture than low salt media WPM and B5 .It is better to add 2.0mgL-16-BA and 1.0 mgL-1GA3 to multiplicationmedium , in which multiplication coefficient reached 4 .Although high rooting percentage was obtained with free growth regulators on 1/2 MS medium , root system development could be improved on medium by adding 0.10 mgL-1 NAA .The survival rate of transplanation was about 80 % in humus soil .Using tap water and edible white sugar instead of distilled water and sucrose have no significant effect on shoots multiplication and growth of H .nepalensis var . sinensis , so the culture cost could be reduced .[ Ch , 6 tab .9 ref .] |
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