| 雷竹不同栽培类型RAPD 分子标记的研究 |
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| 引用本文： | 方伟,何祯祥,黄坚钦,史钱均,林新春. 雷竹不同栽培类型RAPD 分子标记的研究[J]. 浙江农林大学学报, 2001, 18(1): 1-5 |
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| 作者姓名： | 方伟 何祯祥 黄坚钦 史钱均 林新春 |
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| 作者单位： | 1.浙江林学院 竹类研究所, 浙江临安 311300;2.南京林业大学 森林资源与环境学院, 江苏 南京 210037 |
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| 摘 要： | 对雷竹的19 个栽培类型及2 个近缘种进行了RAPD 分析。从4 组80 个引物中筛选出10 个引物, 产生随机扩增位点66 个, 其中多态性位点54 个(占81.8 %)。利用POPGEN 32程序进行聚类分析, 结果表明:①聚类结果可将雷竹的不同栽培类型及其近缘种区分开来,以前认为是雷竹一栽培型的永嘉雷竹不属于Ph .praecox 种系;②细叶雷竹与阔叶雷竹的产量高低主要取决于经营管理;③RAPD 分子标记对竹类植物种以下等级进行分类有一定的可靠性。图2 表3 参8
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| 关 键 词： | 雷竹 随机扩增多态性DNA 分子标记 栽培类型 |
| 收稿时间： | 2000-11-27 |
Studies on cultivars of Phyllostachys pxaecox with RAPD molecular markers |
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| FANG Wei,HE Zheng-xiang,HUANG Jian-qin,SHI Qian-jun,LIN Xin-chun. Studies on cultivars of Phyllostachys pxaecox with RAPD molecular markers[J]. Journal of Zhejiang A&F University, 2001, 18(1): 1-5 |
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| Authors: | FANG Wei HE Zheng-xiang HUANG Jian-qin SHI Qian-jun LIN Xin-chun |
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| Affiliation: | 1.Bamboo Research Institute , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;2.College of Forest Resources and Environment, Nanjiang Forestry University , Nanjiang 210037, Jiangsu, China |
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| Abstract: | With RAPD molecular markers , 19 cultivars and 2 affinis species of Phyllostachys praecox were analysed .Using 10 primers screened from 80 primers , 66 loci were amplified , and 54 of these were polymorphic (81.8 %).The UPGMA cluster analysis of POPGEN 32 program showed :(1)The cultivars and affinis species of Ph .praecox can be divided by UPGMA analysis , and the Yongjia Lei Bamboo recognized as one of the cultivars of Lei Bamboo before doesnt belong to Ph .praecox .(2)The production of narrow-leaved Lei Bamboo and broadleaved Lei Bamboo mainly decided by management . (3)The classification on bamboo under species based on RAPD markers is somewhat reliable . |
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