| 木霉菌对草坪草病原菌的抑菌效果及其机理初步研究 |
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| 引用本文： | 章武,杨锦玉,卢翔,林金梅,牛学礼. 木霉菌对草坪草病原菌的抑菌效果及其机理初步研究[J]. 草业学报, 2021, 30(9): 137-149. DOI: 10.11686/cyxb2020333 |
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| 作者姓名： | 章武 杨锦玉 卢翔 林金梅 牛学礼 |
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| 作者单位： | 岭南师范学院，广东 湛江 524048 |
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| 基金项目： | 广东省自然科学基金项目(2020A1515010238);广东省普通高校重点科研平台和项目(2019KZDXM067);岭南师范学院科研处19年校级人才专项(1170919629);岭南师范学院人才专项(ZL1610) |
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| 摘 要： | 木霉菌是自然界中普遍存在的拮抗微生物,多种木霉菌对植物病原菌具有重要的生防价值.为挖掘出防治草坪草病害的生防菌剂,采用对峙培养法测定了4株木霉菌株对14种草坪草病原菌的拮抗作用,通过显微镜观察、玻璃纸法及粗提液抑菌试验研究了其抑菌机理.研究结果表明,供试木霉菌对所有供试病原菌均有拮抗作用,其中菌株SQ-1Q-18抑菌效...
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| 关 键 词： | 木霉菌 草坪草病原菌 抑菌效果 拮抗机制 哈茨木霉 |
| 收稿时间： | 2020-07-14 |
| 修稿时间： | 2020-10-10 |
A preliminary study of the antifungal activity and antagonism mechanisms of Trichoderma spp. against turfgrass pathogens |
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| ZHANG Wu,YANG Jin-yu,LU Xiang,LIN Jin-mei,NIU Xue-li. A preliminary study of the antifungal activity and antagonism mechanisms of Trichoderma spp. against turfgrass pathogens[J]. Acta Prataculturae Sinica, 2021, 30(9): 137-149. DOI: 10.11686/cyxb2020333 |
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| Authors: | ZHANG Wu YANG Jin-yu LU Xiang LIN Jin-mei NIU Xue-li |
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| Affiliation: | Lingnan Normal University，Zhanjiang 524048，China |
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| Abstract: | Trichoderma spp.， is an antagonistic group of microorganisms widely found in nature and many species have important biocontrol value for use against plant pathogens. In order to evaluate Trichoderma spp. as a potential biocontrol agent for turfgrass disease， the antagonistic effects of four Trichoderma strains against 14 turfgrass pathogens were determined by agar confrontation culture and the mechanism of inhibition was studied by microscopic observation， the ‘cellophane method’ and bacteriostatic testing of crude extract. The results showed that tested Trichoderma spp. isolates had antagonism against all tested pathogens. Among the tested Trichoderma spp. isolates， an isolate designated SQ-1Q-18 had the greatest antibacterial efficacy. The antagonistic activity against Rhizoctonia zeae and Laetisaria fuciformis was scored as level I， with 100% inhibition rate； for Botrytis cinerea， Colletotrichum hainanese and Rhizoctonia solani the antagonistic activity was scored as level Ⅱ， with over 80% inhibition rate for the most potent Trichoderma isolate， and an average inhibition rate against these three pathogens of up to 72.8%. Further study of the inhibitory mechanism of the SQ-1Q-18 isolate on 14 pathogens was that Trichoderma surrounded the growth space of pathogens by generating a bacteriostatic circle， and covered and inserted into the pathogen colony and entwined with it to make the pathogen mycelium become thin， constricted and even broken. Trichoderma could also penetrate directly into a pathogen mycelium to absorb nutrients and cause mycelium cytolysis. In addition， the SQ-1Q-18 isolate could produce antagonistic compounds with inhibitory activity against the turfgrass pathogens. The antagonistic substances produced by the cellophane culture method had strong antagonistic effects against Sclerotinia homoeocarpa， Nigrospora sphaerica and L. fuciformis， and the relative inhibitory rate was as high as 91.77%， 89.80% and 79.59%， respectively. The inhibition rate of Trichoderma crude extract against S. homoeocarpa and Bipolaris peregianensis was 43.60% and 42.91%， respectively. Furthermore， the SQ-1Q-18 isolate was identified as Trichoderma harzianum by morphological characteristics and ITS-rDNA sequence. This study provides scientific data for the research and development of a biocontrol agent for efficient control of turfgrass fungal diseases. |
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| Keywords: | Trichoderma spp. turfgrass pathogens antibacterial activity antagonistic mechanism Trichoderma harzianum |
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