| 崂山奶山羊DGAT1基因多态性及与泌乳性状的关系 |
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| 引用本文： | 王桂芝,李成渤,秦孜娟,王建民,戈新,赵金山,李培培,王建华. 崂山奶山羊DGAT1基因多态性及与泌乳性状的关系[J]. 中国农业科学, 2010, 43(22): 4717-4724. DOI: 10.3864/j.issn.0578-1752.2010.22.020 |
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| 作者姓名： | 王桂芝 李成渤 秦孜娟 王建民 戈新 赵金山 李培培 王建华 |
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| 作者单位： | (山东农业大学动物科技学院) |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 【目的】探讨崂山奶山羊DGAT1基因与泌乳性状的关系。【方法】以175只崂山奶山羊泌乳母羊为试验群体,利用PCR-SSCP方法和候选基因直接测序法检测DGAT1-P1(包含第8外显子)、DGAT1-P2(5’调控区内)、DGAT1-P3和DGAT1-P4位点在该群体中的多态性,并采用最小二乘方法分析DGAT1-P4位点多态性与泌乳性状之间的关联效应。【结果】崂山奶山羊DGAT1-P1和DGAT1-P2位点不存在多态性。对崂山奶山羊高乳脂率和低乳脂率个体的DGAT1-P3位点核苷酸序列比对,发现在序列600bp处即DAGT1-P4位点上存在着A→G的单核苷酸替换,对乳脂率有显著影响(P0.01),其中AA基因型为崂山奶山羊乳脂率性状最有利的基因型,A等位基因对乳脂率性状有正效应。【结论】在崂山奶山羊中发现候选基因DGAT1存在多态性,且对乳脂率性状有显著效应,为开展崂山奶山羊的标记辅助选择提供了依据。
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| 关 键 词： | 崂山奶山羊 DGAT1基因 泌乳量 乳脂率 乳蛋白率 |
| 收稿时间： | 2010-05-19; |
Genetic Polymorphism of DGAT1 Gene Association with Lactation Traits in Laoshan Dairy Goat |
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| WANG Gui-zhi,LI Cheng-bo,QIN Zi-juan,WANG Jian-min,GE Xin,ZHAO Jin-shan,LI Pei-pei,WANG Jian-hua. Genetic Polymorphism of DGAT1 Gene Association with Lactation Traits in Laoshan Dairy Goat[J]. Scientia Agricultura Sinica, 2010, 43(22): 4717-4724. DOI: 10.3864/j.issn.0578-1752.2010.22.020 |
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| Authors: | WANG Gui-zhi LI Cheng-bo QIN Zi-juan WANG Jian-min GE Xin ZHAO Jin-shan LI Pei-pei WANG Jian-hua |
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| Affiliation: | (College of Animal Science and Technology, Shandong Agricultural University)
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| Abstract: | 【Objective】 The present study was aimed to research the association of DGAT1 candidate gene with milk raits in Laoshan dairy goat. 【Method】 Diacylglycerol acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final and only committed step in the synthesis of triglycerides. PCR-single strand conformation polymorphism (PCR-SSCP) assay was used to diagnose polymorphisms of DGAT1-P1(including exon 5), DGAT1-P2 (containing 5′ control region) and DGAT1-P4 loci in dairy goat populations (containing 175 individuals). Polymorphism of DGAT1-P3 locus was analyzed by direct sequencing of candidate gene, and least square method was performed to evaluate the association of DGAT1-P4 site with milk traits. 【Result】 No polymorphism was found in DGAT1-P1 and DGAT1-P2 sites. A mutation from A to G at 600 bp of DGAT1-P3 was found by means of comparative between DNAs in high fat rate and low fat rate goats. One A→G single nucleotide polymorphism was identified at DAGT1-P4 site, which had a significant effect on the fat rate (P＜0.01), and the genotype AA was the most favorable one, the alleles A had a positive effect on fat rate. 【Conclusion】 Results from this study show that the candidate gene DGAT1 has a diversity in Laoshan dairy goat and a significant effect on milk fat rate trait, so they could provide valuable genetic markers for marker-assisted selection of Laoshan dairy goat. |
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| Keywords: | Laoshan dairy goat diacylglycerol acyltransferase 1 gene milk yield fat rate protein rate |
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