| 醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定 |
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| 引用本文： | 任璐,刘晓峰,周建波,殷辉,张宝俊,赵晓军. 醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定[J]. 草地学报, 2021, 29(3): 434-442. DOI: 10.11733/j.issn.1007-0435.2021.03.003 |
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| 作者姓名： | 任璐 刘晓峰 周建波 殷辉 张宝俊 赵晓军 |
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| 作者单位： | 山西农业大学植物保护学院, 山西 太原 030031 |
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| 基金项目： | 山西省应用基础研究计划项目;山西省重点研发计划项目 |
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| 摘 要： | 为明确醉鱼草(Buddleja lindleyana Fortune)内生细菌ZJ1的抑菌作用及其抑菌代谢物质组成,本研究测定了Z J1菌株及其发酵液的抑菌作用,分离、纯化及鉴定了Z J1菌株的抗菌物质,还观察和测定了Z J1菌株代谢物处理后的Botrytis cinerea菌丝生长状态和细胞膜通透性.结果表明:ZJ1...
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| 关 键 词： | 醉鱼草 内生细菌 抑菌脂肽 表面活性素 泛革素 |
| 收稿时间： | 2020-10-04 |
Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites |
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| REN Lu,LIU Xiao-feng,ZHOU Jian-bo,YIN Hui,ZHANG Bao-jun,ZHAO Xiao-jun. Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites[J]. Acta Agrestia Sinica, 2021, 29(3): 434-442. DOI: 10.11733/j.issn.1007-0435.2021.03.003 |
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| Authors: | REN Lu LIU Xiao-feng ZHOU Jian-bo YIN Hui ZHANG Bao-jun ZHAO Xiao-jun |
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| Affiliation: | College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi Province 030031, China |
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| Abstract: | To identify antifungal action of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and its antimicrobial metabolites qualitative composition,the tablet confrontation and growth rate method were used to determine the antifungal action of ZJ1 strain and its fermentation broth to 10 kinds of pathogenic fungi.Medium pressure chromatography was used to isolate and purify the crude extracts of antifungal substances from ZJ1 strain,and LC-MS was used to identify the crude extracts by mass spectrometry.The growth status and membrane permeability of B.cinerea mycelia treated with ZJ1 fermentation broth were measured by microscopic observation and electrical conductivity method.The results showed that ZJ1 strain had certain inhibition to all the 10 fungal pathogens,among them,the inhibition rate of ZJ1 strain and its fermentation broth to M.fructicola were highest,reached 95.69%and 94.68%.The physical characteristics of metabolites of ZJ1 strain were measured and it was found that the metabolites of ZJ1 strain had the ability of discharging oil and the droplet collapse phenomenon occurred.Further tests showed that the metabolites of lipids in ZJ1 strain contained surfactant and ubiquitin.With B.cinerea as indicator pathogenic fungi treated with metabolites of ZJ1 strain,B.cinerea mycelia rarely sporulated,grew abnormally and fractured and the membrane permeability of B.cinerea increased,the permeable material flowed out,the electrical conductivity increased.The endophytic bacteria ZJ1 showed superior antifungal effect,and the identification of its peptide metabolites provided the basis for the further development and utilization of ZJ1 strain. |
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| Keywords: | Buddleja lindleyana Fortune Endophytic Bacteria Antagonistic lipopeptide Surfactin Fengyic |
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