| 山核桃水溶性成分中神经营养活性因子的分离与鉴定 |
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| 引用本文： | 吴建峰,高 飞,谢 倩,黄坚钦,钱永常. 山核桃水溶性成分中神经营养活性因子的分离与鉴定[J]. 中国农学通报, 2018, 34(15): 159-164. DOI: 10.11924/j.issn.1000-6850.casb17040076 |
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| 作者姓名： | 吴建峰 高 飞 谢 倩 黄坚钦 钱永常 |
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| 作者单位： | 浙江农林大学林业与生物技术学院,浙江农林大学林业与生物技术学院,浙江农林大学林业与生物技术学院,浙江农林大学林业与生物技术学院,浙江农林大学林业与生物技术学院 |
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| 基金项目： | 浙江省省院合作林业科技项目“山核桃神经功能因子挖掘与产品开发的研究”(2014SY06)；浙江省自然科学基金项目“蟾酥中抗肿瘤活性 多肽的分离、鉴定及其抗肿瘤机制的研究”(LQ14H280007)；浙江农林大学科研发展基金“天然产物活性因子筛选平台的建立”(2012FR017)。 |
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| 摘 要： | 本研究旨在揭示山核桃水溶性成分的神经营养活性及活性因子。利用脱脂山核桃水溶性提取物处理SH-SY5Y细胞，用倒置相差显微镜观察细胞突触生长，用qPCR检测相关基因表达，使用HPLC及LC-MS对山核桃水溶性成分神经营养活性因子进行分离鉴定。结果显示，山核桃的水溶性成分能够显著促进SH-SY5Y细胞的突触生长，并可以显著上调SH-SY5Y细胞NF160 基因的表达。HPLC及LCMS检测结果发现其水溶性成分主要组成为胞苷、尿苷、鸟苷、腺嘌呤、腺苷等。由此可知，山核桃水溶性成分通过上调NF160基因表达具有神经营养活性。
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| 关 键 词： | 昼夜温度变化 昼夜温度变化 Bt棉 杀虫蛋白 棉铃虫 死亡率 |
| 收稿时间： | 2017-04-12 |
| 修稿时间： | 2017-06-08 |
Isolation and Identification of Neurotrophic Factors from Water-soluble Constituents of Carya cathayensis Sarg. |
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| Abstract: | [Objective] To study the neurotrophic activity of water-soluble constituents from hickory and identify functional factors of it. [Methods] The SH-SY5Y cell was treated with a non-fat hickory water-soluble extract, outgrowth of neurites was detected by microscope and expression of related genes was measured by qPCR method, isolation and identify of neurotrophic factor was carried by HPLC and LC-MS method. [Results] The results showed that hickory water-soluble extract could promote the neurites outgrowth of SH-SY5Y significantly, as well as the gene expression of NF160. The results of HPLC and LC-MS showed that the main constituents of hickory water-soluble are cytoside, urea, guanosine, adenine and adenosine. [Conclusion] It is concluded that hickory water-soluble constituents have neurotrophic activity by manipulate the gene expression of NF160. |
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| Keywords: | Hickory, Water-soluble constituents, SH-SY5Y cells, HPLC LC-MS, Neurotrophic activity |
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