| 秸秆还田配施氮肥对中南地区水稻土壤细菌群落结构多样性的影响 |
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| 引用本文： | 冯晓赟,牛巧龙,李洁,张贵龙,赖欣,常泓. 秸秆还田配施氮肥对中南地区水稻土壤细菌群落结构多样性的影响[J]. 中国农学通报, 2018, 34(11): 66-73. DOI: 10.11924/j.issn.1000-6850.casb16090007 |
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| 作者姓名： | 冯晓赟 牛巧龙 李洁 张贵龙 赖欣 常泓 |
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| 作者单位： | 农业部环境保护科研监测所;山西农业大学生命科学院,农业部环境保护科研监测所,农业部环境保护科研监测所,农业部环境保护科研监测所,农业部环境保护科研监测所,山西农业大学期刊社 |
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| 基金项目： | 国家科技支撑计划项目“中南城郊环保型高效农业模式研究与示范”(2014BAD14B05)；农业部引进国际先进农业科学技术项目“农田土壤 碳汇计量监测技术体系引进与应用”(2015-Z7)，“循环农业管理计量模型和分析方法引进与应用”(2016-X53)。 |
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| 摘 要： | 为探讨作物秸秆与化学氮肥配施对水稻土壤细菌群落多样性的影响，研究在大田中设置单施梯度量氮肥、秸秆还田与梯度量氮肥配施等处理，以不施加秸秆及氮肥处理为对照，采用PCR-DGGE克隆测序技术，分析比较在水稻各生育期内不同处理样品的细菌群落结构、多样性指数和丰富度指数等的变化。试验结果表明，在水稻苗期、分蘖期和拔节期秸秆配施氮肥处理的丰富度指数、多样性指数总体高于单施氮肥处理，而在水稻灌浆期和成熟期秸秆配施氮肥处理的丰富度指数、多样性指数总体低于单施氮肥处理；秸秆配施氮肥处理和单施氮肥处理的丰富度指数、多样性指数在水稻生育期内随施氮量的增加分别呈现不同的变化趋势，而均匀度指数没有显著差异；对各时期土壤样品DGGE图谱进行分析，发现在水稻分蘖期、灌浆期和成熟期各特异性条带主要属于‘变形菌门’的‘假平胞菌属’、‘放线菌门’的‘链霉菌属’；、‘β-变形菌纲’的‘丛毛单胞菌科’、‘α-变形菌纲’的‘鞘氨醇单胞菌科’、‘酸杆菌门’、‘甲基单胞菌科’、‘ε-变形菌纲’、‘硝化螺旋菌属’、‘壁菌门’等。
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| 关 键 词： | 农业 农业 气象灾害 食用杏 产量 影响评估 |
| 收稿时间： | 2016-09-02 |
| 修稿时间： | 2018-03-19 |
Effects of Straw Returning Combined with Nitrogen Fertilizer on Paddy Soil Bacterial Community Diversity in Central South Region of China |
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| Abstract: | The aim is to study the effects of straw returning combined with nitrogen fertilizer on paddy soil bacterial community diversity. Different treatments in field were set as following: gradient amount of nitrogen fertilizer (U), straw returning combined with gradient amount of nitrogen fertilizer (SU), no straw return and no nitrogen fertilizer as the control. The soil bacterial community structure, diversity index and richness index of different treatments in different rice growing stages were analyzed and compared by using PCR-DGGE cloning and sequencing technology. The results showed that the diversity index and richness index of SU treatment were higher than that of U treatment at seedling stage, tillering stage and elongation stage. On the contrast, these indexes of SU treatment were lower than that of U treatment at filling stage and maturation stage. With the nitrogen fertilizer increasing, the diversity index and richness index of SU and U treatments showed different trends, however the evenness index showed no significant variation. Meanwhile, through DGGE fingerprint analysis, the specific bands appeared at the tillering stage, filling stage and maturity stage weremainly identified as Sphingomonas belonging to Proteobacteria, Streptomyces sp. belonging to Actinobacteria, Comamonadaceae belonging to Betaproteobacteria, Sphingomonadaceae belonging to Alphaproteobacteria, Acidobacteria, Methylomonadaceae, Epsilonproteobacteria, Nitrospira and Firmicutes, etc. |
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| Keywords: | straw returning PCR-DGGE soil bacteria bacterial diversity cloning and sequencing |
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