| 鸡肉和鸡肝中有机磷农药残留的检测 |
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| 引用本文： | 郑姗姗,LI Chong-jiu,毕陶桃. 鸡肉和鸡肝中有机磷农药残留的检测[J]. 中国畜牧兽医, 2008, 35(8): 146-149 |
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| 作者姓名： | 郑姗姗 LI Chong-jiu 毕陶桃 |
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| 作者单位： | 1.中国农业大学理学院，北京 100193； 2.中国农业科学院北京畜牧兽医研究所，北京 100193 |
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| 摘 要： | 试验研究了鸡肌肉和肝脏组织中10种有机磷农药残留的气相色谱－火焰光度检测方法。用丙酮和二氯甲烷混合溶剂提取，磷酸缓冲溶液沉淀蛋白质等，凝胶色谱法去除剩余油脂等，用配有火焰光度检测器的气相色谱仪检测，外标法定量。在0.05、0.2、1 mg/kg添加水平，肌肉组织的平均添加回收率在74.2%～108.0％，变异系数在1.6%～17. 3%之间。肝脏组织的平均添加回收率在69.8%~103.0％，变异系数在1.0%～16.0 %之间。
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| 关 键 词： | 有机磷 气相色谱－火焰光度检测 残留 鸡组织 |
Determination of Organophosphorus Pesticide Residues in Muscle and Liver of Chicken by Capillary Gas Chromatography-flame Photometric Detector |
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| ZHENG Shan-shan,LI Chong-jiu,BI Tao-tao. Determination of Organophosphorus Pesticide Residues in Muscle and Liver of Chicken by Capillary Gas Chromatography-flame Photometric Detector[J]. China Animal Husbandry & Veterinary Medicine, 2008, 35(8): 146-149 |
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| Authors: | ZHENG Shan-shan LI Chong-jiu BI Tao-tao |
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| Affiliation: | 1. College of Science, China Agriculture University, Beijing 100193, China;2. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China |
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| Abstract: | A method for the determination of organophosphorus pesticide residues in muscle and liver of chicken by GC FPD was described in this paper. Samples were extracted with acetone/ dichloromethane (3∶4) mixture, added PBS(pH=7.4) to deposit protein. Some fats and hydrophobic impurities were removed by gel permeation chromatography (GPC). The sample was analyzed by flame photometric detector (FPD), through a capillary column of DB 17. The residues were quantified by external standard method. In this method the recoveries(spiked at 0.05, 0.2, and 1 mg/kg) were 74.2%～108.0％ for muscle, 69.8%~103.0％ for liver. Coefficients of variation were 1.6%～17. 3% for muscle, 1.0%～16.0 % for liver, respectively. |
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| Keywords: | organophosphorus pesticide GC-FPD residues chicken tissue |
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