| 不同浓度赤霉酸对饲料添加菌生长的影响 |
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| 引用本文： | 麦提图尔荪·阿卜杜克热木,布沙热木·阿布力孜,乌斯满·依米提. 不同浓度赤霉酸对饲料添加菌生长的影响[J]. 畜牧与饲料科学, 2019, 40(9): 50-53. DOI: 10.12160/j.issn.1672-5190.2019.09.010 |
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| 作者姓名： | 麦提图尔荪·阿卜杜克热木 布沙热木·阿布力孜 乌斯满·依米提 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆 乌鲁木齐 830046 |
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| 基金项目： | 国家自然科学基金项目“混合菌添加对青贮饲料发酵品质及消化率的影响”（31660014） |
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| 摘 要： | 为研究不同浓度的赤霉酸对饲料添加菌生长的影响,用2种不同浓度的赤霉酸溶液单独添加饲料乳酸菌（A4+A7）和纤维素分解菌（Nf+Y6）进行培养52 h,其中每4 h作一个单位测定出OD600 nm值并绘制生长曲线,分析不同浓度的赤霉酸对饲料添加菌生长的影响。结果表明,赤霉酸浓度为10 mg/L时,各组OD600 nm值分别为0.64、0.70、0.84、0.78、0.72,其中试验组2的OD600 nm值与对照组和其他试验组相比有明显增高,总活菌数高达11.6×108 CFU/mL,比对照组（1.63×108 CFU/mL）高7倍以上;当赤霉酸浓度增加到20 mg/L时,各组OD600 nm值分别为0.64、0.60、0.59、0.59、0.63,其中各试验组的OD600 nm值与对照组相比无明显差异（P>0.05）,试验组活菌数（1.60×108 CFU/mL）与对照组相比（1.63×108 CFU/mL）无明显差异（P>0.05）。通过试验数据和生长曲线得知赤霉酸浓度在10 mg/L时能促进乳酸菌和纤维素分解菌的生长繁殖;赤霉酸浓度为20 mg/L时乳酸菌和纤维素分解菌的生长速度明显下降。综上提示,适当添加赤霉酸对饲料添加菌生长有明显的促进作用,赤霉酸浓度过高则饲料添加菌的生长量降低。
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| 关 键 词： | 赤霉酸 乳酸菌 纤维素分解菌 青贮饲料 |
| 收稿时间： | 2019-07-30 |
Effects of Different Concentrations of Gibberellic Acid on Growth of Bacteria Added in Feed Additives |
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| Maitituersun,#xb,Abudukeremu,Busharemu,#xb,Abulizi,Wusiman,#xb,Yimiti. Effects of Different Concentrations of Gibberellic Acid on Growth of Bacteria Added in Feed Additives[J]. Animal Husbandry and Feed Science, 2019, 40(9): 50-53. DOI: 10.12160/j.issn.1672-5190.2019.09.010 |
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| Authors: | Maitituersun Abudukeremu Busharemu Abulizi Wusiman Yimiti |
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| Affiliation: | College of Life Science and Technology,Xinjiang University,Urumqi 830046,China |
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| Abstract: | In order to assess the effects of different concentrations of gibberellic acid on the growth of bacteria added in feed additives, two different concentrations （10 mg/L and 20 mg/L） of gibberellic acid solutions were added to the culture medium of lactic acid bacteria strains of A4+A7 and cellulose-decomposing bacteria strains of Nf+Y6 separately for a 52-hour-long cultivation. The OD600 nm value was determined every 4 hours and the growth curve was drawn. The results showed that when the adding concentration of gibberellic acid was 10 mg/L, the OD600 nm values of the different groups were 0.64, 0.70, 0.84, 0.78 and 0.72, respectively. The OD600 nm value of the experimental group 2 were obviously higher than that of the control group and the other experimental groups. The total viable count of bacteria in experimental group 2 was 11.6×108 CFU/mL, which was 7 times higher than that of the control group （1.63×108 CFU/mL）. When the concentration of gibberellic acid increased to 20 mg/L, the OD600 nm values of the different groups were 0.64, 0.60, 0.59, 0.59 and 0.63, respectively. There was no significant （P>0.05） difference in OD600 nm values between the experimental groups and control group, and there was no significant （P>0.05） difference in total viable count of bacteria between the experimental group （1.60×108 CFU/mL） and control group （1.63×108 CFU/mL）. The experimental data and growth curve showed that the growth of lactic acid bacteria and cellulose-decomposing bacteria could be promoted when the concentration of gibberellic acid was 10 mg/L, while the growth rate of lactic acid bacteria and cellulose-decomposing bacteria was decreased significantly when the concentration of gibberellic acid was 20 mg/L. The combined data suggested that proper addition of gibberellic acid could obviously promote the growth of the bacteria added in feed additives, but the growth of bacteria was inhibited when the concentration of gibberellic acid was exorbitant. |
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| Keywords: | gibberellic acid lactic acid bacteria cellulose-decomposing bacteria silage |
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