The Association Analysis of Prolactin Receptor Gene Polymorphisms with Milk Yield in Yili Horse |
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| Authors: | YU Xi ZANG Chang-jiang LIU Ling-ling MA Hai-yu HE Mei-sheng JIANG Xin CAO Hang LIU Wu-jun |
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| Affiliation: | College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | This experiment was designed to analyze association of prolactin receptor (PRLR) gene polymorphism with milk yield of Yili horse.The number of 60 horse were selected as samples, detected genetic polymorphism of PRLR gene using PCR-SSCP technique and sequencing technology, and then analyzed the polymorphism with milk yield of Yili horse.The results showed that there were two polymorphism fragments of PRLR gene flanking region, there were three genotypes:AA, AB and BB.Two novel SNPs (g.29764513 G>A and g.30106804 C>A) were identified of PRLR gene flanking region by sequencing.The g.29764513 G>A SNP caused amino acid variations as p.33His>Arg, while the g.30106804 C>A SNP was synonymous mutation without causing amino acid changes.Statistical results indicated that the g.29764513 G>A and g.30106804 C>A SNPs were significantly associated with daily milk yield in Yili horse (P<0.05).These mutations might be as a crucial DNA genetic markersfor milk production traits selection in Yili horse. |
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| Keywords: | Yili horse PRLR gene milk yield |
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