Cloning and Sequence Analysis of A6L Gene of Goatpox Virus |
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| Authors: | ZHAO Yin-long WU Guo-hua ZHU Hai-xia WU Jian YAN Xin-min ZHAO Zhi-xun LI Jian WU Na MU Xiao-feng ZHANG Qiang |
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| Affiliation: | Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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| Abstract: | To explore the molecular characteristics of protein A6 from goatpox virus (GTPV), genomic DNA was extracted from goatpox virus Gulang isolate.The specific primers were designed and used to amplify the A6L gene from the genomic DNA by PCR.A PCR product was ligated into pGEM-T Easy vector.After transformation into E.coli DH5α, the positive clones were sequenced and the sequences were analyzed with the bioinformatics softwares.The result showed that A6L gene sequence contained an open reading frame (ORF) of 1128 nucleotides and deduced protein consisted of 375 amino acids with the theoretical molecular weight of 43.68 ku and isoelectric point of 7.50.Analysis of secondary structure of protein A6 revealed that α-helix, β-strand and random coil were 53.30%, 10.24% and 39.46%, respectively.Analysis of multiple sequence alignment indicated A6L gene from different capripoxvirus isolates were highly conserved with identity of more than 97%.The present results laid the foundation for further studies of biological functions of protein A6 and interaction among the early proteins of capripoxvirus. |
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| Keywords: | goatpox virus A6L gene gene cloning sequence analysis |
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