| 氮营养对青花菜花球酚类物质及抗氧化能力的影响 |
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| 引用本文： | 李 娟,栗娜娜,郭世荣,李 军. 氮营养对青花菜花球酚类物质及抗氧化能力的影响[J]. 植物营养与肥料学报, 2008, 14(6): 1123-1128. DOI: 10.11674/zwyf.2008.0615 |
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| 作者姓名： | 李 娟 栗娜娜 郭世荣 李 军 |
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| 作者单位： | 1.南京农业大学园艺学院,江苏南京 210095 |
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| 摘 要： | 采用无机基质盆栽,研究不同氮营养水平（5、10、15和20 mmol/L）对两品种青花菜花球生长、总酚、类黄酮含量及抗氧化能力的影响。结果表明,提高氮浓度水平,有利于青花菜株高、花球直径、花球鲜重和干重的增加;两品种花球的总酚含量随着氮浓度的增加呈降低的变化趋势,当供氮浓度高于10 mmol/L,总酚含量显著降低。花球中主要含有槲皮素和山萘黄素两种黄酮醇物质,而且山萘黄素含量是槲皮素的1.48倍;在低氮 （5 mmol/L）处理下,两品种花球的槲皮素含量和绿优2号的山萘黄素含量显著高于其他处理。增加供氮浓度,花球的二苯代苦味酰基（DPPH, 1,1-diphenyl-2-picrylhydrazyl）自由基清除率和铁还原抗氧化能力(FRAP, Ferric reducing /antioxidant power assay) 值呈下降趋势,限制了其抗氧化能力。相关性分析表明,两品种花球的FRAP值与酚类物质的正相关性显著高于DPPH自由基清除率。两品种相比较,中晚熟品种马拉松的花球直径、鲜重和干重显著高于中早熟品种绿优2号,而其总酚含量和抗氧化能力明显低于绿优2号。以上结果说明,通过氮营养水平能够调控青花菜酚类物质合成和抗氧化能力,从而为生产高营养品质的青花菜产品提供了可能的途径。
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| 关 键 词： | 青花菜 氮 总酚 类黄酮 抗氧化能力 |
| 收稿时间： | 2007-10-22 |
Effects of different nitrogen supply level on the phenolic content and antioxidant activity in broccoli head |
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| LI Juan LI Na-na GUO Shi-rong LI Jun. Effects of different nitrogen supply level on the phenolic content and antioxidant activity in broccoli head[J]. Plant Nutrition and Fertilizer Science, 2008, 14(6): 1123-1128. DOI: 10.11674/zwyf.2008.0615 |
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| Authors: | LI Juan LI Na-na GUO Shi-rong LI Jun |
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| Affiliation: | 1.College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China |
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| Abstract: | The effects of different nitrogen supply level (5, 10, 15 and 20 mmol/L) on the head growth, total phenolic, the flavonoids contents and antioxidant activity were investigated in two broccoli (Brassica olerecea var. italica) cultivars (middle late maturity cv. Malasong and middle early maturity cv. Lüyou No.2 ) by inorganic medium culture. The results showed that plant height, head diameter, head fresh and dry weight increased significantly with increasing nitrogen applied. However, total phenolic content decreased with increasing nitrogen supply, especially when applied nitrogen level was higher than 10 mmol/L. Quercetin and kaempferol were identified in broccoli head, and kaempferol content was 1.48 times higher than quercetin content, and their contents reached highest level when applied nitrogen concentration was 5 mmol/L. Increasing nitrogen supply decreased DPPH radical scavenging and FRAP value, and markedly inhibited the antioxidant acitivity in broccoli head when nitrogen supply level was 20 mmol/L. A significantly positive correlation was found between FRAP value and the phenolic content in two cultivars. Compared with two cultivars, the head growth of cv. Malasong was better than that of cv. Lüyou No.2, but was contrary to total phenolic and antioxidant activity. These results clearly demonstrated the phenolic substances and antioxidant activity in broccoli were strongly affected by fertilizer regimes especially nitrogen application level. |
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| Keywords: | broccoli nitrogen total phenolic flavonoid antioxidant activity |
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