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桂果蔗1号不同级数健康种苗生长特征及性状变异
引用本文:刘红坚,梁文燊,何为中,叶权,刘丽敏,何毅波,卢曼曼,刘俊仙,李松,林善海. 桂果蔗1号不同级数健康种苗生长特征及性状变异[J]. 南方农业学报, 2021, 52(2): 288-296. DOI: 10.3969/j.issn.2095-1191.2021.02.003
作者姓名:刘红坚  梁文燊  何为中  叶权  刘丽敏  何毅波  卢曼曼  刘俊仙  李松  林善海
作者单位:广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/农业农村部广西甘蔗生物技术与遗传改良重点实验室,南宁530007;广西北海嘉桓农业科技有限公司,广西北海 536000;合浦县生产力促进中心,广西北海 536100
基金项目:广西创新驱动发展专项资金项目(桂科AA17202042-3);广西重点研发项目(桂科AB19245028);广西农业科学院稳定资助团队项目(桂农科2018YT04);广西农业科学院基本科研业务专项(桂农科2017YM05)
摘    要:【目的】评价桂果蔗1号不同级数健康种苗的生长特征和变异特性,为提高果蔗健康种苗生产用种质量及高产栽培提供科学依据。【方法】对桂果蔗1号组培苗、第一级种茎和第二级种茎苗期生长表型性状进行连续观测,分析3个不同级数果蔗幼苗的苗数、株高、地径、叶片数及+1叶的叶长、叶宽和叶面积等7个表型性状的生长特征和变异稳定性。【结果】不同级数果蔗种苗生长特征差异明显。第一级种茎和第二级种茎各方面生长特征相近,且均显著优于组培苗(P< 0.05),但第二级种茎各方面表现仍明显优于第一级种茎。桂果蔗1号健康种苗各性状平均变异系数为5.48%,变异幅度为3.01%~11.88%,变异幅度最大的是苗数,最小的是地径。除了第二级种茎+1叶叶宽变异幅度有所上升外,其余6项表型性状变异均伴随着级数的增加而趋向稳定。3个不同级数种源所有性状的平均变异系数为3.32%~8.25%,以组培苗的平均变异系数最大(8.25%),第一级种茎苗次之(4.88%),第二级种茎苗最小(3.32%)。说明组培苗性状分离最大,高级苗(第二级种茎)性状较稳定。【结论】桂果蔗1号健康种苗第二级种茎的生长特性综合表现显著优于第一级种茎和组培苗,遗传变异也较稳定,为生产栽培最佳用种。

关 键 词:果蔗  健康种苗  生长特征  遗传变异
收稿时间:2020-04-03

Growth characters and properties variation of different generations of chewing cane Guiguozhe 1 seedlings
LIU Hong-jian,LIANG Wen-shen,HE Wei-zhong,YE Quan,LIU Li-min,HE Yi-bo,LU Man-man,LIU Jun-xian,LI Song,LIN Shan-hai. Growth characters and properties variation of different generations of chewing cane Guiguozhe 1 seedlings[J]. Journal of Southern Agriculture, 2021, 52(2): 288-296. DOI: 10.3969/j.issn.2095-1191.2021.02.003
Authors:LIU Hong-jian  LIANG Wen-shen  HE Wei-zhong  YE Quan  LIU Li-min  HE Yi-bo  LU Man-man  LIU Jun-xian  LI Song  LIN Shan-hai
Affiliation:1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi), Ministry of Agriculture and Rural Affairs, Nanning 530007, China;2. Beihai Jiahuan Agricultural Technology Co., Ltd., Beihai, Guangxi 536000, China;3. Hepu Productivity Promotion Center, Beihai, Guangxi 536100, China
Abstract:【Objective】Evaluating the growth characters and genetic characters of seedlings regenerated from healthy cane setts from different generations of chewing cane cultivar Guiguozhe 1 could help improve the quality of healthy cane setts and high-yielding cultivation.【Method】Phenotypic characters of seedlings from three different generations of Guiguozhe 1 including tissue culture seedling(TCS), first generation seedling(FGS) and second generation seedling(SGS) were successively measured.Growth characters and variability stability of seven traits in seedlings from three generations were analyzed, including number of seedling, plant height, ground diameter, number of leaves, and leaf length, leaf width and area of the first leaf.【Result】Growth characters were obviously different among generations.Phenotypic characters of FGS were close to SGS, and they performed better than TCS(P< 0.05), but the performance of SGS was still better than FGS.Variation coefficient average value of 7 growth characters in seedlings from three generations was 5.48%.The variance range was 3.01%-11.88%, the number of seedling had the maximum variation and the ground diameter had the minimum variation.Except for the increase in variance of the first leaf width in SGS, the variances of other six growth characters tended to stabilize along with the increasing of generations.The variance coefficient average value of all seven characters ranged from 3.32% to 8.25%, that of TCS was the largest(8.25%), FGS was in middle(4.88%) and SGS was the smallest(3.32%).The results suggest that the characters segregation of TCS was the maximum, and the variance was relatively stable in later-generation(SGS).【Conclusion】The SGS of chewing cane cultivar Guiguozhe 1 is comprehensively superior to TCS and FGS for all the growth characters, and its genetic variance is stable.SGS is recommended as the best cane setts for cultivation.
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