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细叶石斛和翅梗石斛花朵赋香成分的GC-MS分析
引用本文:王元成,曾艺芸,李振坚,刘月新,马秀红,孙振元. 细叶石斛和翅梗石斛花朵赋香成分的GC-MS分析[J]. 林业科学研究, 2020, 33(3): 116-123
作者姓名:王元成  曾艺芸  李振坚  刘月新  马秀红  孙振元
作者单位:中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091;北京市天竺苗圃,北京100621
基金项目:引进国际先进林业科学技术项目项目);中央级公益性科研院所基本科研业务费专项
摘    要:[目的]本研究明确2种浓香型石斛花朵的香气成分及其含量,探明其香气组成,以期为石斛花的保健开发提供参考。[方法]采用顶空固相微萃取(HS-SPME)结合气相色谱-质谱联用(GC-MS)技术,分析了2种浓香型石斛花朵的挥发性成分。[结果]发现细叶石斛花含挥发性成分52个,翅梗石斛花含挥发性成分35个,主要成分皆为烯烃类。[结论]首次测定了翅梗石斛新鲜花朵的挥发性成分,其特征香气成分为罗勒烯、β-石竹烯和d-柠檬烯。细叶石斛花的特征香气成分为罗勒烯、β-石竹烯、芳樟醇。

关 键 词:兰科  石斛  花香  挥发性成分  气质联用  固相微萃取

Aroma Constituents in Flower of Dendrobium hancockii and D.trigonopus Based on SPME-GC-MS Analysis
WANG Yuan-cheng,ZENG Yi-yun,LI Zhen-jian,LIU Yue-xin,MA Xiu-hong,SUN Zhen-yuan. Aroma Constituents in Flower of Dendrobium hancockii and D.trigonopus Based on SPME-GC-MS Analysis[J]. Forest Research, 2020, 33(3): 116-123
Authors:WANG Yuan-cheng  ZENG Yi-yun  LI Zhen-jian  LIU Yue-xin  MA Xiu-hong  SUN Zhen-yuan
Affiliation:(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,Beijing 100091,China;Tianzhu Nursery,Beijing 100621,China)
Abstract:[Objective]To analyze the aroma components and the relative contents in the flowers of two Dendrobium species,D.hancockii and D.trigonopus.[Method]The volatile constituents and their contents were analyzed by solid-phase microextraction(SPME)and gas chromatography-mass spectrometry(GC-MS).[Result]For the first time,the volatile constituents were analyzed in fresh flowers of D.trigonopus and thirty-five compounds were characterized.Fifty-two compounds were characterized in D.hancockii.The main volatile constituent in both the two species was olefin.[Conclusion]The characteristic aroma components are(Z)-β-Ocimene,β-Caryophyllene and Linalool in D.hancockii flower.The characteristic aroma components are(Z)-β-Ocimene,β-Caryophyllene and d-(+)-Limonene in D.trigonopus flower.
Keywords:Orchidaceae  Dendrobium  floral scent  volatile constituents  GC-MS  SPME
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