| 德钦’紫花苜蓿叶绿体基因组序列及特征分析 |
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| 引用本文： | 孙志轩,敖平星,毕玉芬,赵雁. 德钦’紫花苜蓿叶绿体基因组序列及特征分析[J]. 草地学报, 2022, 30(2): 320-328. DOI: 10.11733/j.issn.1007-0435.2022.02.008 |
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| 作者姓名： | 孙志轩 敖平星 毕玉芬 赵雁 |
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| 作者单位： | 1. 云南农业大学园林园艺学院, 云南 昆明 650201;2. 云南农业大学动物科学技术学院, 云南 昆明 650201 |
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| 基金项目： | 云南农业大学第十四届学生科技创新创业行动基金项目;国家自然科学基金 |
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| 摘 要： | ‘德钦’紫花苜蓿(Medicago sativa L. ‘Deqin’，以下简称‘德钦’苜蓿)是一种优良的地方品种，具有特异耐旱性、耐热性和耐酸铝性。本研究利用Illumina第二代高通量测序技术对其叶绿体全基因组进行测序(NCBI登录号：MN218692)，并对其结构及特征进行分析。结果显示：‘德钦’苜蓿叶绿体基因组含有一个反向重复区，属于IR缺失型；总长为125 470 bp，共有110个基因，其中蛋白质编码基因76个、tRNA 30个和rRNA 4个，缺失3个基因(rps16，rpl22和infA)，总GC含量为33.9%；相对同义密码子使用度显示，‘德钦’苜蓿70.74%的密码子使用度>1，偏好以A和T结尾；共鉴定出115个SSRs位点，其中clpP基因鉴定出与紫花苜蓿不同SSRs位点；最大似然树结果显示‘德钦’苜蓿与紫花苜蓿聚在一起，支持率为75%。研究结果将对苜蓿属叶绿体基因组工程研究及‘德钦’苜蓿特异基因的进一步鉴定利用提供一定的参考价值。
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| 关 键 词： | 德钦’紫花苜蓿 叶绿体基因组 高通量测序 特征分析 系统发育分析 |
| 收稿时间： | 2021-07-16 |
Complete Chloroplast Genome Sequence and Characteristics Analysis of Medicago sativa'Deqin' |
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| SUN Zhi-xuan,AO Ping-xing,BI Yu-fen,ZHAO Yan. Complete Chloroplast Genome Sequence and Characteristics Analysis of Medicago sativa'Deqin'[J]. Acta Agrestia Sinica, 2022, 30(2): 320-328. DOI: 10.11733/j.issn.1007-0435.2022.02.008 |
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| Authors: | SUN Zhi-xuan AO Ping-xing BI Yu-fen ZHAO Yan |
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| Affiliation: | 1. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China |
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| Abstract: | Medicago sativa‘Deqin’(hereinafter referred to as‘Deqin’)is an excellent alfalfa local variety with strong drought,heat,and aluminum-acid toxicity resistance.In this study,the whole chloroplast genome of‘Deqin’was sequenced by Illumina second-generation high-throughput sequencing technology(NCBI accession number:MN218692),and its structure and characteristics were analyzed.The results showed that the chloroplast genome of‘Deqin’,with a total length of 125470 bp,has one inverted repeat region and belongs to IR lacking clad.A total of 110 coding genes,including 76 protein-coding genes,30 tRNAs and 4 rRNAs,and 3 genes(rps16,rpl22 and infA)are absent,with an overall GC content of 33.9%.Relative synonymous codon usage showed that 70.74%of codons RSCU>1 in‘Deqin’,with preference ending with A and T.SSRs analysis identified 115 SSRs loci.Different SSRs loci from‘Deqin’were identified in the gene of clpP.The phylogenetic tree shows that‘Deqin’and M.sativa L.were clustered into one group with 75%support.The results have reference value for Medicago chloroplast genome engineering and further identification and utilization of specific genes of‘Deqin’. |
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| Keywords: | Medicago sativa‘Deqin’ Chloroplast genome Characteristic analysis High-throughput sequencing Phylogenetic analysis |
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