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| 牛源皮特不动杆菌对小鼠的致病性分析 | |
| 引用本文： | 樊利虹,郭红瑞,吴江,易军,马晓平,苟丽萍,谢跃,叶刚,左之才. 牛源皮特不动杆菌对小鼠的致病性分析[J]. 浙江农业学报, 2021, 33(2): 230-238. DOI: 10.3969/j.issn.1004-1524.2021.02.06 |
| 作者姓名： | 樊利虹 郭红瑞 吴江 易军 马晓平 苟丽萍 谢跃 叶刚 左之才 |
| 作者单位： | 1.四川农业大学 动物医学院,四川 温江 6111302.深圳海关动植物检验检疫技术中心,广东 深圳 5180453.四川省畜牧科学研究院,四川 成都 610066 |
| 基金项目： | 四川省科技计划(2018NZ0002);四川省科技计划(2019YFQ0012);国家重点研发计划(2018YFD0501800);国家现代农业产业技术体系四川肉牛创新团队项目(SCCXTD-2020-13) |
| 摘 要： | 为探究来源于牛呼吸道疾病综合征发病牛的皮特不动杆菌对小鼠的致病性,对其进行相关毒力基因的检测和小鼠致病性试验,试验内容主要包括临床症状观察、病理剖检、组织病理学观察,以及半数致死量(LD50)和各器官载菌量的测定.结果显示,牛源皮特不动杆菌ZZCNC1807-6和ZZCSF1807-9菌株对小鼠均具有较强的致死性,对小... |
| 关 键 词： | 皮特不动杆菌 毒力基因 半数致死量 组织病理学 |
| 收稿时间： | 2020-08-03 |
Pathogenicity of Acinetobacter pittii from beef cattle in mice |
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| FAN Lihong,GUO Hongrui,WU Jiang,YI Jun,MA Xiaoping,GOU Liping,XIE Yue,YE Gang,ZUO Zhicai. Pathogenicity of Acinetobacter pittii from beef cattle in mice[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 230-238. DOI: 10.3969/j.issn.1004-1524.2021.02.06 | |
| Authors: | FAN Lihong GUO Hongrui WU Jiang YI Jun MA Xiaoping GOU Liping XIE Yue YE Gang ZUO Zhicai |
| Affiliation: | 1. College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China 2. Inspection and Quarantine Center for Animals and Plants, Shenzhen Customs, Shenzhen 518045, China 3. Sichuan Academy of Animal Husbandry, Chengdu 610066, China |
| Abstract: | In order to explore the pathogenicity of two strains of Acinetobacter pittii from bovine respiratory disease complex (BRDC) infected beef cattle to mice, the related virulence genes were detected and the pathogenicity test of mice was carried out, which mainly included clinical symptom observation, pathological examination, histopathological observation, and determination of median lethal dosage (LD50) and bacterial load in various organs. The results showed that ZZCNC1807-6 and ZZCSF1807-9 stains were highly lethal to mice, and their LD50 reached 4.487×107 CFU·mL-1 and 3.177×108 CFU·mL-1, respectively. After pathology autopsy, it was seen that Acinetobacter pittii mainly caused subcutaneously hyperemia, lung congestion and swelling, intestinal pneumatosis and effusion and other symptoms in mice. And it could cause infection of many organs, such as heart, liver, spleen, lung, kidney and brain. Histopathological observation showed that the pathological changes of various organs in mice were mild, mainly manifested in a small amount of inflammatory cells infiltration in liver and lung, and necrosis and fibrous hyperplasia of white pulp cells in spleen. Among them, the pathological changes of mice in ZZCNC1807-6 group were more serious than those in ZZCSF1807-9 group. The analysis of bacterial load showed that the invasiveness of the two strains to the liver, heart, spleen, lung and kidney of mice had little difference, but ZZCNC1807-6 strain had stronger invasiveness to the brain than ZZCSF1807-9. To sum up, the virulence of strain ZZCNC1807-6 was stronger than that of ZZCSF1807-9. |
| Keywords: | Acinetobacter pittii virulence genes median lethal dose histopathology |
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