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The effect of photoperiod and feeding frequency on larval of the Amazonian ornamental fish Pyrrhulina brevis (Steindachner, 1876)
Authors:Galileu Crovatto Veras  Marcos Ferreira Brabo  Joel Artur Dias  Higo Andrade Abe  Zélia Maria Pimentel Nunes  Luis David Solis Murgas
Affiliation:1. Faculty of Fisheries Engineering, Institute of Coastal Studies at the Federal University of Pará, Bragan?a, Pará, Brazil;2. Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil
Abstract:This study aimed to evaluate the performance of Pyrrhulina larvae, Pyrrhulina brevis, under different photoperiods and feeding frequencies. A completely randomized design was used, with four replicates in a 5 × 2 factorial, with five photoperiods (6L:18D, 12L:12D, 16L:8D, 20L:4D, 24L:0D) and two feeding frequencies (two or four times a day). Four hundred larvae with an initial length of 4.6 ± 0.09 mm were randomly distributed in 40, 1‐L containers at a density of 10 larvae/L. Feeding was performed with Artemia nauplii in the ratio of 150 Artemia nauplii/larva/feeding. After 15 days, all larvae were euthanized for measurements of length (mm), final weight (mg), survival rate (%) and larvae uniformity in weight an length (%). The data were subjected to analysis of variance and subsequently, in case of significance, a Tukey test was performed to compare means. There was no interaction between photoperiod and feeding frequency on studied variables (P > 0.05). Likewise, there was no influence of feed rate on the same variables (P > 0.05). There was only influence of photoperiod, where the larvae subjected to longest photoperiods (24L:0D and 20L:4D) showed improved weight and length (P < 0.05). Therefore, a photoperiod of 20L:4E, with feeding twice a day is recommended.
Keywords:   Artemia     cycle of light/dark  feeding management  fish larvae  ornamental fish
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