| 两种基因型番茄对镉胁迫响应差异 |
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| 引用本文： | 赵首萍,张永志,张棋,王钢军,叶雪珠. 两种基因型番茄对镉胁迫响应差异[J]. 植物营养与肥料学报, 2015, 21(5): 1261-1268. DOI: 10.11674/zwyf.2015.0520 |
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| 作者姓名： | 赵首萍 张永志 张棋 王钢军 叶雪珠 |
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| 作者单位： | 1.浙江省食品安全重点实验室,浙江省农业科学院农产品质量标准研究所,杭州 310021 |
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| 摘 要： | 【目的】使用两个番茄品种YSL189高镉积累和HZ903低Cd积累进行苗期水培实验,旨在揭示番茄品种间Cd耐性差异的机理,为分子育种改良番茄重金属耐性提供新的信息。【方法】本研究以前期鉴定的番茄高Cd积累品种YSL189和低Cd积累品种HZ903为材料,采用苗期人工气候箱水培的方式,分析了在Cd 50和100μmol/L胁迫下,供试品种间形态学指标、抗氧化酶活性、Cd积累量及相关基因表达的差异,同时分析了供试品种苗期对离子态Cd的吸收速率差异。【结果】1)在Cd 50和100μmol/L胁迫下,根长对Cd胁迫最敏感,株高和生物量次之;与低Cd积累品种HZ903相比,高Cd积累品种YSL189表现出生物量大、根系短、株高较高的特点。2)在50和100μmol/L Cd胁迫下,植株Cd积累量以根茎叶的趋势递减;同等胁迫下,YSL189植株根、茎、叶部的Cd积累量都显著高于HZ903。3)当培养介质中Cd浓度大于20μmol/L时,YSL189根系对离子态Cd的吸收速率显著高于HZ903;定量PCR结果显示,在50和100μmol/L Cd胁迫下,品种间Nramp2、Nramp3和ZIP的表达量差异趋势与Cd积累的差异趋势一致,即YSL189根系Cd吸收相关基因Nramp2,Nramp3和ZIP的表达量高于HZ903。4)在Cd 50和100μmol/L胁迫下,根、茎部的SOD(超氧化物歧化酶)、POD(过氧化物酶)和CAT(过氧化氢酶)活性都是YSL189高于HZ903,同时YSL189叶部POD和SOD活性也显著高于HZ903,同时,TBARS(巴比妥酸反应底物)含量所指示的根、茎部膜质过氧化伤害程度则没有品种间差异。【结论】YSL189根系对离子态Cd的高吸收速率以及Nramp2、Nramp3和ZIP基因的高表达量,可能是其获得高镉积累量的原因之一;同等胁迫条件下,YSL189植株体内高Cd浓度诱导了抗氧化酶的高活性,这说明YSL189植株对活性氧的清除能力强于HZ903。
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| 关 键 词： | 镉 番茄 吸收转运 抗氧化酶 基因型差异 |
| 收稿时间： | 2014-04-21 |
Differential responses of two tomato cultivars to cadmium stress |
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| ZHAO Shou-ping,ZHANG Yong-zhi,ZHANG Qi,WANG Gang-jun,YE Xue-zhu. Differential responses of two tomato cultivars to cadmium stress[J]. Plant Nutrition and Fertilizer Science, 2015, 21(5): 1261-1268. DOI: 10.11674/zwyf.2015.0520 |
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| Authors: | ZHAO Shou-ping ZHANG Yong-zhi ZHANG Qi WANG Gang-jun YE Xue-zhu |
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| Affiliation: | 1.Zhejiang Province Key Lab for Food Safety/Institute of Quality and Standard for Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China |
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| Abstract: | 【Objectives】To investigate the different responses of Cd accumulation in tomato cultivars exposed to Cadmium stress, a hydroponics experiment at seedling stage was performed using two tomato varieties, YSL189 with high Cd accumulation and HZ903 with low Cd accumulation, which were confirmed by our previous research. 【Methods】The experiment was performed in growth chamber, and morphological index, antioxidant enzyme activity, differences in Cd accumulation, ionic absorption rate and related gene expression in varieties were analyzed using seedling exposed to Cd 50 and 100 μmol/L. 【Results】 When exposed to 50 and 100 μmol/L of Cd, the root length of plants was more sensitive to Cd stress than plant height and biomass. Cultivar YSL189 exhibited higher tolerance in biomass and plant height, but lower root length than HZ903. Under both 50 and 100 μmol/L Cd treatments, the accumulation of Cd was reduced significantly in the order of roots, stem and leaves accordingly, and the Cd concentration in roots, stem and leaves of YSL189 was significantly higher than in those of HZ903. When the concentration of Cd in culture medium was over 20 μmol/L, the uptake rate of Cd in the roots of YSL189 was significantly higher than that in the roots of HZ903. The results of Real-Time PCR proved that the expression levels of transporter genes Nramp2, Nramp3 and ZIP in the roots of YSL 189 were higher than those in the roots of HZ903, and higher activities of SOD(superoxide dismutase), POD(peroxidase) and CAT(atalase) in roots and stems, SOD and POD in leaves were higher in YSL189 than those in HZ903, but no significant difference in concentration of TBARS(thiobarbituric acid reactive substance) between cultivars was found, indicating the degree of lipid peroxidation. 【Conclusion】 The higher Cd accumulation in YSL189 may be partly attributed to the higher Cd uptake rate, expression levels of Nramp2, Nramp3, and ZIP in roots of YSL189 were higher than those of HZ903; and the higher activities of antioxidant enzymes in YSL189 were induced by higher Cd concentration, indicating the stronger power to detoxify reactive oxygen species for YSL189 than HZ903. |
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