Aluminium tolerance in lentil (Lens culinaris Medik.) with monogenic inheritance pattern |
| |
| Authors: | Dharmendra Singh Harsh Kumar Dikshit Arun Kumar |
| |
| Affiliation: | 1. Division of Genetics, Indian Agricultural Research Institute, New Delhi, India;2. National Phytotron Facility, Indian Agricultural Research Institute, New Delhi, India |
| |
| Abstract: | The objectives of this study were to determine genetics of Al tolerance and whether the Al tolerance observed is governed by the same gene. The lines ‘L‐7903’ and ‘L‐4602’ have been developed through breeding programme as Al‐tolerant lines. These lines showed maximum root regrowth and minimum accumulation of Al and callose as compared to sensitive genotypes (‘BM‐4’ and ‘L‐4147’). Al tolerance in the parents, F1, F2 and backcross generations was estimated using the regrowth of the primary root after staining and scoring of fluorescent signals. The F1 hybrids responded similarly to the tolerant parents, indicating dominance of Al tolerance over sensitivity. The segregation ratios obtained for Al tolerance and sensitivity in the F2 and backcross generations were 3 : 1 and 1 : 1, respectively. Test of allelism confirmed the same gene was conferring Al tolerance in both genotypes (‘L‐7903’ and ‘L‐4602’) as the F1 was also tolerant and no segregation of tolerant : sensitive was recorded. These results indicated that Al tolerance is a monogenic dominant trait that can be easily transferred to agronomic bases through backcross breeding technique. |
| |
| Keywords: |
Lens culinaris
aluminium tolerance inheritance callose accumulation root regrowth |
|
|
