Genetic characterization of group A acetylsaponin‐deficient mutants from wild soybean (Glycine soja Sieb. and Zucc.) |
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| Authors: | Panneerselvam Krishnamurthy Jeong‐Dong Lee Bo‐Keun Ha Jong‐Hyun Chae Jong Tae Song Chigen Tsukamoto Ram J. Singh Gyuhwa Chung |
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| Affiliation: | 1. Department of Biotechnology, Chonnam National University, Yeosu, Chonnam, Korea;2. Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), Jeonju, Korea;3. School of Applied Biosciences, Kyungpook National University, Daegu, Korea;4. Division of Plant Biotechnology, Chonnam National Unviersity, Gwangju, Korea;5. Department of Applied Biological Chemistry, Iwate University, Morioka, Iwate, Japan;6. USDA/ARS, National Soybean Research Laboratory, University of Illinois, Urbana, IL, USA |
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| Abstract: | Group A acetylsaponins are the main causative components for bitter and astringent tastes of soybean (Glycine max). In this study, we examined the genetic nature of the absence of group A acetylsaponins in 12 Korean wild soybean (Glycine soja) accessions. In all 12 accessions, the coding region (1431‐bp) of Sg‐1 locus was identical with Sg‐1a, which adds the xylose sugar moiety at the terminal position of the C‐22 sugar chain of SS‐A, except one nucleotide (G→A change) at +948th position. This point mutation results in change of one amino acid from tryptophan (TGG) to stop codon (TGA). We observed that the mutated Sg‐1 was controlled by a single recessive gene (sg‐10‐a1). This gene was mapped between BARCSOYSSR_07_1561 and BARCSOYSSR_07_1598 on soybean chromosome 7. Our study demonstrated that the mutated Sg‐1 gene in Korean wild soybeans is genetically different from those identified in Japanese soybean cultivar ‘Kinusayaka’ and wild soybean JP‐36121. We believe that the new Sg‐1 mutants can also be utilized to produce a new soybean variety without bitter and astringent properties. |
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| Keywords: | group A saponins C‐22 sugar chain biosynthesis soyasaponins soybean breeding
Glycine soja
wild soybean |
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