首页 | 本学科首页   官方微博 | 高级检索  
     

黄颡鱼感染小肠结肠炎耶尔森氏菌的组织病理学观察
引用本文:赵静,王利. 黄颡鱼感染小肠结肠炎耶尔森氏菌的组织病理学观察[J]. 中国畜牧兽医, 2014, 41(12): 254-257
作者姓名:赵静  王利
作者单位:1. 西南民族大学青藏高原研究院, 四川成都 610041;2. 西南民族大学生命科学与技术学院, 四川成都 610041
基金项目:四川省科技支撑项目(2013FZ0014);西南民族大学研究生学位点建设项目(2013XWD-S071007).
摘    要:为探讨小肠结肠炎耶尔森氏菌GM2402株对黄颡鱼的致病性,本试验采用人工回感试验测定其半数致死浓度(LC50),同时采用组织病理学方法探讨该菌对黄颡鱼组织的影响.结果表明:回感18 h后,发病黄颡鱼腹部肿大,胸鳍、腹鳍基部出血,肛门红肿,其LC50为3.0×106.2 CFU/mL.组织病理学观察肝细胞肿大、排列紊乱、广泛空泡变性;肾小球萎缩,肾小管上皮细胞肿大;脾脏组织结构疏松充满大量红细胞;鳃小片轻度水肿充血.超微结构观察结果显示肝脏和肠道细胞内线粒体均出现不同程度的肿胀,嵴断裂囊泡化.本试验结果表明小肠结肠炎耶尔森氏菌GM2402株对黄颡鱼有较强的致病性,可导致鱼体多个组织出现病变.

关 键 词:黄颡鱼  小肠结肠炎耶尔森氏菌  组织病理学  
收稿时间:2014-07-01

Histopathological Observation of Pelteobagrus fulvidraco Infected with Yersinia enterocolitica
ZHAO Jing,WANG Li. Histopathological Observation of Pelteobagrus fulvidraco Infected with Yersinia enterocolitica[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(12): 254-257
Authors:ZHAO Jing  WANG Li
Affiliation:1. Institute of Qinghai-Tibet Plateau, Southwest University for Nationalities, Chengdu 610041, China;2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
Abstract:The healthy Pelteobagrus fulvidraco were artificially infected with Yersinia enterocolitica (Y.enterocolitica) strain GM2402 by intraperitoneal injection to test the median lethal concentration (LC50).The histology changes of Pelteobagrus fulvidraco were studied by histopathological methods.These results showed that the challenged fish indicated abdomen swelling, pectoral fin and ventral fin bleeding, swelling and bleeding on anus after intraperitoneal injection.The LC50 value of Y.enterocolitica in Pelteobagrus fulvidraco was 3.0×106.2 CFU/mL.The main histopathological changes of organs indicated that swelling and degeneration of hepatocyte were observed in the liver.In the kidney, swelling and degeneration were showed in renal tubule epithelial cell.A large number of erythrocyte emerged in spleen. Gill lamella showed mild congestion and swollen.The ultrastructures indicated that there were distinct changes in liver and intestine, especially in mitochondria.The cristaes of mitochondria were disordered and disappeared. In conclusion, Y.enterocolitica strain GM2402 was pathogenic to Pelteobagrus fulvidraco.
Keywords:Pelteobagrus fulvidraco  Yersinia enterocolitica  histopathology  
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号