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杨树湿地松组织培养愈伤组织耐盐性*
引用本文:张立钦,郑勇平,罗士元,胡加共. 杨树湿地松组织培养愈伤组织耐盐性*[J]. 浙江农林大学学报, 1997, 14(1): 16-21
作者姓名:张立钦  郑勇平  罗士元  胡加共
作者单位:1.浙江林学院林学系, 临安 311300;2.浙江省林业厅种苗站;3.浙江省台州地区林业特产局
基金项目:* 浙江省自然科学基金资助项目
摘    要:对杨树无性系N L-80105, N L-80106, N L-80117和湿地松组织培养愈伤组织的耐盐性进行了研究。结果表明:以MS为基本培养基,添加2,4-D 2.0 mg kg-1和KT 0.5 mg kg-1,诱导这些树种愈伤组织效果好;在含氯化钠的培养基上,杨树不同器官外植体的愈伤组织诱导能力和耐盐程度有差异,外植体茎、叶比根强;杨树继代愈伤组织的耐盐能力显著高于湿地松,这与植株水平的耐盐性相一致,表明用愈伤组织鉴别这些植物耐盐性的方法是可行的。

关 键 词:杨属   无性系   湿地松   组织培养   愈伤组织   耐盐性
收稿时间:1996-01-25

Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures
Zhang Liqin,Zheng Yong ping,Luo Shiyuan,Hu Jiagong.. Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures[J]. Journal of Zhejiang A&F University, 1997, 14(1): 16-21
Authors:Zhang Liqin  Zheng Yong ping  Luo Shiyuan  Hu Jiagong.
Affiliation:1.Zhejiang Forestry College, Lin ’an 311300, PRC
Abstract:The tolerance to NaCl of tissues culture calli of Populus clones (NL-80105, NL-80106, and NL-80117) and Pinus elliottii was studied. The results were as follow s: (1) MS medium with 2.0 mg kg- 1 of 2, 4-D and 0.5 mg kg- 1 KT was suitable for tree calli in tissuecul tures; (2) the salt tolerances of calli from roots, stems and petioles were different on MS containing NaCl. The calli with the highest salt to lerance was from stems; (3) the salt tolerance of subculture calli of Populus clones was higher than that of Pinus elliottii, which was in keeping with the salt tolerance of Populus and Pinus elliottii trees.
Keywords:
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