| 盐酸环丙沙星在患子宫内膜炎奶牛子宫内给药的药物浓度 |
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| 引用本文： | 陶金忠,杨国林,李剑勇,李世宏,赵兴绪,严作庭,谢家声,巩忠福. 盐酸环丙沙星在患子宫内膜炎奶牛子宫内给药的药物浓度[J]. 中国畜牧兽医, 2008, 35(5): 68-71 |
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| 作者姓名： | 陶金忠 杨国林 李剑勇 李世宏 赵兴绪 严作庭 谢家声 巩忠福 |
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| 作者单位： | 1.宁夏大学农学院，银川 750021；2.甘肃农业大学，兰州 730070；3.中国农业科学院兰州畜牧与兽药研究所，兰州 730050 |
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| 摘 要： | 本研究以反相高效液相色谱为定量分析手段，选用5头患子宫内膜炎的奶牛，通过子宫内灌注盐酸环丙沙星（2.5 g/头），研究了盐酸环丙沙星在患子宫内膜炎奶牛体内的药物动力学规律。以二氟沙星为内标，血浆样品经甲醇沉淀蛋白，离心，经针头式过滤器处理，用反相高效液相法测定其中盐酸环丙沙星的浓度。 色谱条件为：ODS-1C18柱；测定流动相为0.015 mol/L四丁基溴化铵溶液-乙腈（92∶8，V/V），pH为3.0；流速为1.0 ml/min；荧光检测器，激发波长（λex）278 nm，发射波长（λem）465 nm。通过采用MCPKP房室分析程序，分析血中浓度 时间数据，发现有3头奶牛血样药时数据符合无吸收三室开放模型。其血样中主要药动学参数为：T1/2α为0.916 h、T1/2β为49.20 h、AUC高达7.6296 mg/L·h、Clβ为1.582 L/kg·h，β为0.642 h-1。有2头奶牛血样药时数据符合一级吸收二室开放模型。其主要药动学参数为：T1/2α为1.26 h、T1/2β为9.2 h、AUC高达28.336 mg/L·h，β为0.3571 h-1。试验结果表明，盐酸环丙沙星子宫给药吸收快，分布广，消除慢。
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| 关 键 词： | 盐酸环丙沙星 药动学 反相高效液相色谱法 子宫内膜炎 奶牛 |
Study of Pharmacokinetics of Ciprofloxacin Hydrochloride in the Blood of Endometritis Dairy Cows |
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| TAO Jin-zhong,YANG Guo-lin,LI Jian-yong,LI Shi-hong,ZHAO Xing-xu,YAN Zuo-ting,XIE Jia-sheng,GONG Zhong-fu. Study of Pharmacokinetics of Ciprofloxacin Hydrochloride in the Blood of Endometritis Dairy Cows[J]. China Animal Husbandry & Veterinary Medicine, 2008, 35(5): 68-71 |
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| Authors: | TAO Jin-zhong YANG Guo-lin LI Jian-yong LI Shi-hong ZHAO Xing-xu YAN Zuo-ting XIE Jia-sheng GONG Zhong-fu |
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| Affiliation: | 1.School of Agriculture of Ningxia University, Yinchuan 750021, China;2.Faculty of Veterinary Medicine of Gansu Agricultural University, Lanzhou 730070, China;3.Lanzhou Institute of Pasturage and Veterinary Medicine of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China |
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| Abstract: | The pharmacokinetics and residues of ciprofloxacin hydrochloride in the milk were investigated in 5 cows suffered from endometritis following single intrauterine administration at the dosage of 2.5 per cow. Blood and milk were sampled at different intervals after administration and ciprofloxacin hydrochloride concentrations were determined by RP-HPLC method with a fluorescent detector. Difloxacin was used as intra-standard. The concentration time data were analyzed with MCPKP program. The ciprofloxacin hydrochloride concentration time data were fitted to a two compartment open model with first order absorption in two cows suffered from endometritis after single intrauterus. The main pharmacokinetics parameters were as follow: T 1/2α 1.26 h, T1/2β9.2 h, AUC 28.336 mg/L·h, β 0.3571 h-1. The ciprofloxacin hydrochloride concentration-time data were fitted to a three-compartment open model with non absorption in other three cows. The main pharmacokinetics parameters were as follow: T 1/2α0.916 h, T1/2β49.20 h, AUC 7.6296 mg/L·h,Clβ 1.582 L/kg·h, β 0.642 h-1. The results showed that ciprofloxaxin hydrochloride was absorbed fast and distributed extensively, but it was eliminated slowly in the blood after single intrauterine administration. |
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| Keywords: | ciprofloxacin hydrochloride pharmacokinetics RP-HPLC endometritis dairy cow |
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