| 小麦品种资源耐盐性鉴定 |
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| 引用本文： | 郑甲成, 尤依锦, 刘婷, 等. 盐胁迫对TaERECTA转基因小麦种子萌发和幼苗生理特性的影响[J]. 云南农业大学学报（自然科学）, 2019, 34(6): 922-927. DOI: 10.12101/j.issn.1004-390X(n).201902006 |
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| 作者姓名： | 郑甲成 尤依锦 刘婷 李杰勤 刘言龙 武德功 李金才 詹秋文 |
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| 作者单位： | 1.安徽科技学院 农学院，安徽 凤阳 233100;2.安徽农业大学 作物抗逆育种与减灾国家地方联合工程实验室，安徽 合肥 230036 |
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| 基金项目： | 农业部国家重点研发计划粮食丰产增效科技创新重点专项子课题（2017YFD0301301）；国家级大学生创新创业训练计划项目（201710879068）；安徽省教育厅高校科学研究项目（KJ2018A0538）；作物抗逆育种与减灾国家地方联合工程实验室开放基金资助项目（NELCOF20190106） |
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| 摘 要： | 目的研究TaERECTA基因超表达后对小麦耐盐性的影响。方法以转TaERECTA基因小麦为材料，分别在芽期和苗期高盐胁迫，测定种子发芽势、发芽率、发芽指数和相对盐害指数，以及植株枯萎数、幼苗抗氧化酶活性和丙二醛(MDA)含量。结果芽期盐胁迫下，与野生型(WT)株系相比，TaERECTA转基因小麦种子发芽势、发芽率和发芽指数较高，相对盐害指数较低(P<0.05)；苗期盐胁迫下，转基因小麦幼苗平均枯萎数较低，抗氧化酶活性较高，且SOD活性显著高于CK株系(P<0.05)。结论一定盐胁迫下，TaERECTA基因超表达可以增强小麦耐盐性，其作用机制可能是TaERECTA激活组织细胞SOD酶活性，其他抗氧化酶相互协同减轻盐胁迫伤害。转基因株系L10相对盐害指数较小，综合抗盐能力较强，可作为下一步深入研究TaERECTA调控小麦耐盐性功能的重要材料。
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| 关 键 词： | 小麦 TaERECTA 耐盐性 种子和幼苗 抗氧化酶 |
| 收稿时间： | 2019-02-15 |
| 修稿时间： | 2019-04-17 |
The receptor-like cytoplasmic kinase STRK1 phosphorylates and activates CatC,thereby regulating H2O2 homeostasis and improving salt tolerance in rice |
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| Jiacheng ZHENG, Yijin YOU, Ting LIU, et al. Effect of Salt Stress on the Seeds Germination and Seedlings Physiological Traits of TaERECTA Transgenic Wheat[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(6): 922-927. DOI: 10.12101/j.issn.1004-390X(n).201902006 |
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| Authors: | Jiacheng ZHENG Yijin YOU Ting LIU Jieqin LI Yanlong LIU Degong WU Jincai LI Qiuwen ZHAN |
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| Affiliation: | 1.College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China;2.National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | PurposeTo investigate the influence of TaERECTA overexpression on salt tolerance of bread wheat.MethodTaERECTA genetically modified (GM) wheat was used to identify the germination energy, germination rate, germination index and relative salt stress index (RSSI) of seeds, and plant wilting numbers, antioxidant enzyme activity and MDA content of fresh seedlings.ResultCompared to control, the seeds of TaERECTA GM plants displayed higher germination energy, germination rate and germination index, whereas lower RSSI under salt stress at germination stage; fresh seedlings of GM plants showed lower plant wilting numbers and higher antioxidant enzyme activity, with significantly higher SOD activity as to control plants (P<0.05).ConclusionThese results suggest that TaERECTA overexpression could improve salt tolerance of bread wheat after salt stress, due to the mechanism of TaERECTA promotion on the SOD activity of organelle, and other antioxidant enzymes synergistically reduce salt stress injury. Transgenic wheat lines L10 showed lower RSSI and integrally stronger ability of salt tolerance, and line L10 can be used as an important material for further investigation of TaERECTA function on salt tolerance of bread wheat. |
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| Keywords: | Triticum aestivum L. TaERECTA salt tolerance seeds and seedlings antioxidant enzyme |
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