| 长吻鮠精巢及精子结构的研究 |
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| 引用本文： | 刘康, 何金钊, 冯鹏霏, 等. 投喂频率和水平对长吻鮠幼鱼消化酶和肝胰脏脂肪代谢酶活性的影响[J]. 云南农业大学学报（自然科学）, 2019, 34(5): 779-784. DOI: 10.12101/j.issn.1004-390X(n).201812039 |
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| 作者姓名： | 刘康 何金钊 冯鹏霏 马华威 罗旭 |
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| 作者单位： | 1.广西壮族自治区水产引育种中心，广西 南宁 530031;2.广西壮族自治区水产科学研究院，广西 南宁 530031 |
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| 基金项目： | 中央财政农业技术推广项目（桂渔牧科201521055）；广西科技基地和人才专项（桂科AD17195016） |
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| 摘 要： | 目的研究不同投喂频率(2次/d、3次/d)和水平(即每日投喂量占试验鱼体重的比例，2%、4%、6%、8%、10%)对长吻鮠幼鱼消化酶和肝胰脏脂肪代谢酶活性的影响，为长吻鮠的人工养殖提供更健康的养殖模式。方法采用SPSS 17.0软件进行双因素方差分析后采用Duncan多重比较检验，显著水平为0.05。结果(1)当每日投喂频率为3次、投喂水平为6%时，长吻鮠幼鱼消化酶中的肠道蛋白酶、肠道淀粉酶和胰蛋白酶的活性最高，显著高于每日投喂频率为2次、投喂水平为10%的试验组以及每日投喂频率为3次、投喂水平为2%和10%的试验组(P<0.05)。(2)当每日喂频率为3次、投喂水平为6%时，肝胰脏脂肪代谢酶中的肉毒碱脂酰转移酶(CACT)、乙酰辅酶A羧化酶(ACC)、脂蛋白酯酶(LPL)和肝脂酶(HL)的活性最高，显著高于每日投喂频率为2次、投喂水平为2%的试验组以及每日投喂频率为3次、投喂水平为8%和10%的试验组(P<0.05)。结论最适宜投喂频率和水平分别是3次/d和6%，更适合长吻鮠幼鱼的健康养殖。
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| 关 键 词： | 长吻鮠 投喂频率 投喂水平 消化酶 脂肪代谢酶 |
| 收稿时间： | 2018-12-13 |
| 修稿时间： | 2019-03-19 |
Sea bass (Dicentrarchus labrax) larvae fed different Artemia, rations: growth,pancreas enzymatic response and development of digestive functions |
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| Kang LIU, Jinzhao HE, Pengfei FENG, et al. Effects of Feeding Frequency and Level on the Digestive Enzymes and Fat Metabolism Enzyme in the Hepatopancreas of Juvenile Leiocassis longirostris[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(5): 779-784. DOI: 10.12101/j.issn.1004-390X(n).201812039 |
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| Authors: | Kang LIU Jinzhao HE Pengfei FENG Huawei MA Xu LUO |
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| Affiliation: | 1.Guangxi Aquaculture Introduction and Breeding Center, Nanning 530031, China;2.Guanxi Academy of Fishery Sciences, Nanning 530031, China |
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| Abstract: | PurposeThe effects of feeding frequency (2 times/day, 3 times/day) and feeding level (the ratio of daily feeding to experimental fish body weight, 2%, 4%, 6%, 8% and 10%, respectively) on digestive enzymes and hepatic pancreatic fat metabolism enzyme activities of juveniles Leiocassis longirostris were studied, which provided a healthier breeding mode for artificial breeding of L. longirostris.MethodTwo-way analysis of variance was performed using SPSS 17.0 software and Duncan multiple comparison test was used. The significant level was 0.05.Result(1) The activities of intestinal protease, intestinal amylase and trypsin of juveniles with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 10% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 2% and 10%, respectively (P<0.05). (2) The activities of fat metabolism enzymes hepatopancreas of carnitine acyl transferase (CACT), acetyl coenzyme A carboxylase (the ACC), lipoprotein ester enzyme (LPL) and hepatic lipase (HL) with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 2% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 8% and 10%, respectively (P<0.05). ConclusionThe most suitable feeding frequency and level are 3 times/d and 6%, respectively, which is more suitable for the healthy breeding juveniles L. longirostris. |
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| Keywords: | Leiocassis longirostris feeding frequency feeding level digestive enzyme fat metabolism enzyme |
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